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Anti-S100 抗体 [4C4.9] (ab4066)

Reviews (12)Q&A (11)References (66)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

Key features and details

  • Mouse monoclonal [4C4.9] to S100
  • Suitable for: IHC-P
  • Reacts with: Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-S100 antibody [4C4.9]
    S100 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4C4.9] to S100

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Human

  • 免疫原

    Full length protein. Purified bovine brain S-100 protein

  • ポジティブ･コントロール

    • IHC-P: Human Melanoma and skin tissue.

  • 特記事項

    This product was changed from ascites to tissue culture supernatant on 29/01/2021. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab4066の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
IHC-P (4)
1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.
特記事項
IHC-P
1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.

ターゲット情報

  • 機能

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

  • 組織特異性

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

  • 配列類似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    • 別名

      • Bpb antibody
      • NEF antibody
      • Protein S100-A1 antibody
      • S-100 protein alpha chain antibody
      • S-100 protein subunit alpha antibody
      • S100 alpha antibody
      • S100 beta antibody
      • S100 calcium binding protein A1 antibody
      • S100 calcium binding protein B antibody
      • S100 calcium-binding protein A1 antibody
      • S100 protein alpha polypeptide antibody
      • S100A antibody
      • s100a1 antibody
      • S100B antibody
      • S100beta antibody
      • S10A1_HUMAN antibody
      see all

    画像

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

      Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)

      Immunohistochemistry of Formalin fixed paraffin embedded human melanoma tissue sections labeling S100 with ab4066 at 1/100. (x10 magnification).

      This image was generated using the ascites version of the product.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
      Immunohistochemistry of Formalin fixed paraffin embedded human skin labeling S100 with ab4066.

      This image was generated using the ascites version of the product.

    参考文献 (66)

    ab4066 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4066 は 66 報の論文で使用されています。

    • Qu H  et al. Deficiency of Mitochondrial Glycerol 3-Phosphate Dehydrogenase Exacerbates Podocyte Injury and the Progression of Diabetic Kidney Disease. Diabetes 70:1372-1387 (2021). PubMed: 33741719
    • Hernández-Díaz N  et al. Parallel evolution of placental calcium transfer in the lizard Mabuya and eutherian mammals. J Exp Biol 224:N/A (2021). PubMed: 33568441
    • Wang Y  et al. Betacellulin regulates peripheral nerve regeneration by affecting Schwann cell migration and axon elongation. Mol Med 27:27 (2021). PubMed: 33794764
    • Omiya H  et al. BMP signaling suppresses Gemc1 expression and ependymal differentiation of mouse telencephalic progenitors. Sci Rep 11:613 (2021). PubMed: 33436697
    • Rinwa P  et al. Demise of nociceptive Schwann cells causes nerve retraction and pain hyperalgesia. Pain 162:1816-1827 (2021). PubMed: 33979318
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-10 of 23 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

     Average
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Melanoma suspicious cutaneous tissue)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Tris/EDTA
    Permeabilization
    Yes - H2O2
    Specification
    Melanoma suspicious cutaneous tissue
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Apr 25 2022

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

     Excellent
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Rat Tissue sections (Cerebellum)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10 mM citrate buffer pH6
    Permeabilization
    No
    Specification
    Cerebellum
    Blocking step
    Normal Donkey Serum (NDS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C
    Fixative
    Paraformaldehyde
    Read More

    DR. Estela Munoz

    Verified customer

    投稿 Mar 15 2021

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (melanoma)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
    Permeabilization
    No
    Specification
    melanoma
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 19 2016

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (skin)
    Antigen retrieval step
    Other
    Permeabilization
    No
    Specification
    skin
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 Dec 14 2015

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

     Inconclusive
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Brain (Hippocampus))
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.1%TritonX-100/PBS
    Specification
    Brain (Hippocampus)
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    投稿 May 14 2015

    Immunohistochemistry (Frozen sections) abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Human Tissue sections (skin)
    Specification
    skin
    Fixative
    Acetone
    Permeabilization
    Yes - 0,1% Saponin / PBS
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    投稿 Feb 27 2012

    Question

    IHC-FoFr with rat peripheral nerve
    sees no staining
    fix: 4% PFA perfused, postfixed with PFA
    no AR
    Ab: 1/100 o/n 4 degree; 1/50 o/n RT
    block: BSA + goat serum
    2nd: goat anti-mouse, OK

    suggested: try an enzymatic AR step

    Read More
    Answer


    As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

    If not, please let me know.

    As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.

    Read More

    Question

    Dear Sir or Madam,

    could you please also provide me with the concentration of your product Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)?

    Best Regards,

    Read More
    Answer

    Thank you for your interest in our products.

    The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

    If I can assist further, please do not hesitate to contact me.

    Read More

    Question

    Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

    Read More
    Answer

    That's great news! Thanks so much for letting us know.

    Please feel free to contact me if you need anything further.

    Read More

    Question

    I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

    Read More
    Answer

    I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

    To check the status of the order please contact our Customer Service team and reference this number.

    Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

    I wish you the best of luck with your research.

    Read More

    1-10 of 23 Abreviews or Q&A

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