Anti-S100 抗体 [4C4.9] (ab4066)

Reviews (10)Q&A (11)References (47)

Key features and details

  • Mouse monoclonal [4C4.9] to S100
  • Suitable for: IHC-P
  • Reacts with: Mouse, Rat, Cow, Human
  • Isotype: IgG2a

製品の概要

  • 製品名

    Anti-S100 antibody [4C4.9]
    S100 一次抗体 製品一覧

  • 製品の詳細

    Mouse monoclonal [4C4.9] to S100

  • 由来種

    Mouse

  • アプリケーション

    適用あり: IHC-Pmore details

  • 種交差性

    交差種: Mouse, Rat, Cow, Human

  • 免疫原

    Full length protein. Purified bovine brain S-100 protein

  • ポジティブ･コントロール

    • Melanoma.

  • 特記事項

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab4066 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/10 - 1/50. Antigen retrieval is not essential but may optimise staining.

ターゲット情報

  • 機能

    Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.

  • 組織特異性

    Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.

  • 配列類似性

    Belongs to the S-100 family.
    Contains 2 EF-hand domains.

  • 細胞内局在

    Cytoplasm.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • Bpb antibody
    • NEF antibody
    • Protein S100-A1 antibody
    • S-100 protein alpha chain antibody
    • S-100 protein subunit alpha antibody
    • S100 alpha antibody
    • S100 beta antibody
    • S100 calcium binding protein A1 antibody
    • S100 calcium binding protein B antibody
    • S100 calcium-binding protein A1 antibody
    • S100 protein alpha polypeptide antibody
    • S100A antibody
    • s100a1 antibody
    • S100B antibody
    • S100beta antibody
    • S10A1_HUMAN antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-S100 antibody [4C4.9] (ab4066)
    ab4066 staining human melanoma by IHC-P.

参考文献 (47)

ab4066 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4066 は 47 報の論文で使用されています。

  • Zhao W  et al. Expression of P2X receptors in the rat anterior pituitary. Purinergic Signal 16:17-28 (2020). PubMed: 31823189
  • Alanazi AM  et al. Liposomal Resveratrol and/or Carvedilol Attenuate Doxorubicin-Induced Cardiotoxicity by Modulating Inflammation, Oxidative Stress and S100A1 in Rats. Antioxidants (Basel) 9:N/A (2020). PubMed: 32079097
  • Scherer A  et al. Distinct Tumor Microenvironments Are a Defining Feature of Strain-Specific CRISPR/Cas9-Induced MPNSTs. Genes (Basel) 11:N/A (2020). PubMed: 32456131
  • Goodman T  et al. Fibroblast growth factor 10 is a negative regulator of postnatal neurogenesis in the mouse hypothalamus. Development 147:N/A (2020). PubMed: 32661019
  • Liu J  et al. Embryonic stem cell microenvironment enhances proliferation of human retinal pigment epithelium cells by activating the PI3K signaling pathway. Stem Cell Res Ther 11:411 (2020). PubMed: 32967731
View all Publications for this product

レビューと Q&A

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レビューを送る 質問を送る

1-10 of 21 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (melanoma)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrite buffer, pH6.0
Permeabilization
No
Specification
melanoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Aug 19 2016

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-S100 antibody [4C4.9]

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (skin)
Antigen retrieval step
Other
Permeabilization
No
Specification
skin
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
Fixative
Formaldehyde
Read More

Abcam user community

Verified customer

投稿 Dec 14 2015

Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-S100 antibody [4C4.9]

 Inconclusive
Abreviews
abreview image
Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Brain (Hippocampus))
Antigen retrieval step
None
Permeabilization
Yes - 0.1%TritonX-100/PBS
Specification
Brain (Hippocampus)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 0.5, 2 · Temperature: RT°C
Fixative
Paraformaldehyde
Read More

Abcam user community

Verified customer

投稿 May 14 2015

Question

IHC-FoFr with rat peripheral nerve
sees no staining
fix: 4% PFA perfused, postfixed with PFA
no AR
Ab: 1/100 o/n 4 degree; 1/50 o/n RT
block: BSA + goat serum
2nd: goat anti-mouse, OK

suggested: try an enzymatic AR step

Read More
Answer


As we discussed, trying an enzymatic antigen retrieval step could help with the signal.

If not, please let me know.

As for the protocol on the datasheet - this was added most likely from a collaborator or customer. Thus, these must be the conditions that were used by them. I still would suggest to do the overnight incubation at 4 degree as this makes more sense.

Read More

Question

Dear Sir or Madam,

could you please also provide me with the concentration of your product Anti-S100 antibody [4C4.9] - Astrocyte Marker (ab4066)?

Best Regards,

Read More
Answer

Thank you for your interest in our products.

The unit size of this antibody (ab4066) is 500 µl and the concentration is not determined since it is not affinity purified - only IgG fraction.

If I can assist further, please do not hesitate to contact me.

Read More

Question

Thank you for sending the replacement antibody. This one worked very well both in vitro using human induced pluripotent stem cells and in vivo using mouse spinal cord sections.

Read More
Answer

That's great news! Thanks so much for letting us know.

Please feel free to contact me if you need anything further.

Read More

Question

I have used this antibody on astrocytes grown in culture in ICC and mousespinal cord via IHC-Fr. In neither application did I see any signal using 1:200 dilution of the antibody; using another antibody to an astrocyte marker protein, GFAP, is was able to detect astrocytes in both applications using the same samples.

Read More
Answer

I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number 1080427.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More

Immunohistochemistry (Frozen sections) abreview for Anti-S100 antibody [4C4.9] - Astrocyte Marker

 Good
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (skin)
Specification
skin
Fixative
Acetone
Permeabilization
Yes - 0,1% Saponin / PBS
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Read More

Abcam user community

Verified customer

投稿 Feb 27 2012

Question

Thanks for the antibody. It works fine the immunostaining. But I still can not get any signal in western blot, although it was reported that it should work in western blot.



Do you have any other recommendations for western blot?

Read More
Answer

Thank you for your reply. After reviewing the customer feedback for ab4066, it appears that the customers who had obtained good WB results were using BSA as a blocking agent. The feedback we had from a customer using 5% milk was that their WB was unsuccessful. It is possible that this antibody may be sensitive to milk and may produce better results when using BSA.

For both ab4066 andab868, you could also try increasing the primary antibody concentration to the range 1:200-1:500 or loading more protein (30-50ug per lane). I hope this helps, please let me know if you need any further assistance.

Read More

Question

I am trying to do Western Blot and ICC for s100, a marker for astrocytes. The antibody which I got from Abcam is ab4066. I did not get any signal for WB and very weak signal for ICC. So I wonder what is the problem. If I still can not get any signal after trouble shooting, which antibodies do you recommend for WB and ICC, respectively?

Read More
Answer

Thank you for contacting us. I am sorry that ab4066 was not producing satisfactory signal in ICC and WB. Our antibody ab868 is rated highly for use in ICC and WB on human, mouse, rat, cow, and pig samples. If you could please provide some additional details about the protocol and samples you are using, I would be happy to assist you further. If ab4066 is not working as stated on the datasheet and you have purchased it in the past six months, I would be happy to send you ab868 as a free of charge replacement. Could you please provide the following information? 1) What is your original order or PO number? 2) What species and sample type were you testing? How were the cells / tissuefixed or lysed? 3) What permeabilization and/ or blocking agents were used? 4) Could you please tell me the dilution and incubation times for the primary and secondary antibodies? 5) It would be very helpful if you could please provide an image of your WB or ICC.

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1-10 of 21 Abreviews or Q&A

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