Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5251] to S100 alpha - BSA and Azide free
- Suitable for: ICC, Flow Cyt, IP, WB
- Reacts with: Mouse, Rat, Human
製品名Anti-S100 alpha antibody [EPR5251] - BSA and Azide free
S100 alpha 一次抗体 製品一覧
製品の詳細Rabbit monoclonal [EPR5251] to S100 alpha - BSA and Azide free
アプリケーション適用あり: ICC, Flow Cyt, IP, WBmore details
種交差性交差種: Mouse, Rat, Human
Synthetic peptide within Human S100 alpha aa 1-100. The exact sequence is proprietary.
Ab247841 is the carrier-free version of ab109384. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab247841 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
保存方法Shipped at 4°C. Store at +4°C. Do Not Freeze.
解離定数（KD 値）KD = 5.00 x 10 -11 M Learn more about KD
Concentration information loading...
Our Abpromise guarantee covers the use of ab247841 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC||Use at an assay dependent concentration.|
|Flow Cyt||Use at an assay dependent concentration.|
|IP||Use at an assay dependent concentration.|
|WB||Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.|
機能Weakly binds calcium but binds zinc very tightly-distinct binding sites with different affinities exist for both ions on each monomer. Physiological concentrations of potassium ion antagonize the binding of both divalent cations, especially affecting high-affinity calcium-binding sites.
組織特異性Highly prevalent in heart. Also found in lesser quantities in skeletal muscle and brain.
配列類似性Belongs to the S-100 family.
Contains 2 EF-hand domains.
- Information by UniProt
- Bpb antibody
- NEF antibody
- Protein S100-A1 antibody
All lanes : Anti-S100 alpha antibody [EPR5251] (ab109384) at 1/1000 dilution
Lane 1 : Rat kidney tissue lysate
Lane 2 : C2C12 cell lysate
Lane 3 : Mouse kidney tissue lysate
Lane 4 : Human brain lysate
Lane 5 : HT-1080 lysate
Lane 6 : Mouse heart tissue lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 11 kDa
This data was developed using ab109384, the same antibody clone in a different buffer formulation.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.