Application
Western blot
Sample
Human Tissue lysate - whole (Ovary)
Loading amount
50 µg
Specification
Ovary
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG (H&L)
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Detection
Detection method
Pierce Supersignal Westfemto
Exposure
10 second(s)
Bands
Specific: 10 kDa
Positive control
beta-actin
Negative control
no primary
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. John Andersen
Verified customer
投稿 Jun 14 2006