Anti-S100 alpha 2/S100A2 抗体 [EPR5392] - BSA and Azide free (ab247877)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5392] to S100 alpha 2/S100A2 - BSA and Azide free
- Suitable for: IHC-P, WB, IP, Flow Cyt (Intra), ICC/IF
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-S100 alpha 2/S100A2 antibody [EPR5392] - BSA and Azide free
S100 alpha 2/S100A2 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR5392] to S100 alpha 2/S100A2 - BSA and Azide free -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WB, IP, Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HACAT, BxPC3, HeLa and A431 cell lysates; IHC: Human skin and human lung carcinoma tissues IP: HaCaT. ICC/IF: A431 (Human epidermoid carcinoma epithelial cell) cells Flow cyto(intra): A431 (Human epidermoid carcinoma epithelial cell) cells
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特記事項
ab247877 is the carrier-free version of ab109494.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
バッファー
pH: 7.2
Constituent: PBS -
キャリア・フリー
はい -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR5392 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
- Anti-S100 alpha 2/S100A2 antibody [EPR5392] (ab109494)
- PE Anti-S100 alpha 2 antibody [EPR5392] (ab306147)
- APC Anti-S100 alpha 2 antibody [EPR5392] (ab306148)
- HRP Anti-S100 alpha 2 antibody [EPR5392] (ab306149)
- Alexa Fluor® 488 Anti-S100 alpha 2 antibody [EPR5392] (ab309858)
- Alexa Fluor® 647 Anti-S100 alpha 2 antibody [EPR5392] (ab310226)
- Alexa Fluor® 594 Anti-S100 alpha 2 antibody [EPR5392] (ab310674)
- Alexa Fluor® 555 Anti-S100 alpha 2 antibody [EPR5392] (ab312204)
- Alexa Fluor® 568 Anti-S100 alpha 2/S100A2 antibody [EPR5392] (ab312693)
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab247877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa.
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IP |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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ICC/IF |
Use at an assay dependent concentration.
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特記事項 |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. |
IP
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
ICC/IF
Use at an assay dependent concentration. |
ターゲット情報
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機能
May function as calcium sensor and modulator, contributing to cellular calcium signaling. May function by interacting with other proteins, such as TPR-containing proteins, and indirectly play a role in many physiological processes. May also play a role in suppressing tumor cell growth. -
組織特異性
A subset of epithelial cells including normal human mammary epithelial cells and keratinocytes. -
配列類似性
Belongs to the S-100 family.
Contains 2 EF-hand domains. -
発生段階
Preferentially expressed in normal human mammary epithelial cells as opposed to tumor-derived ones. The level of S100L was shown to correlate inversely with tumor progression. - Information by UniProt
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参照データベース
- Entrez Gene: 6273 Human
- Omim: 176993 Human
- SwissProt: P29034 Human
- Unigene: 516484 Human
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別名
- CAN19 antibody
- MGC111539 antibody
- Protein S 100L antibody
see all
画像
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This data was developed using ab109494, the same antibody clone in a different buffer formulation.
Intracellular Flow Cytometry analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling S100 alpha 2 with purified ab109494 at 1/20 dilution (10 ug/ml) (Red). Cells were fixed with 80% Methanol and permeabilised with 0.1% Tween-20. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150081) (1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as a isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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This data was developed using ab109494, the same antibody clone in a different buffer formulation.
Immunocytochemistry/ Immunofluorescence analysis of A431 (Human epidermoid carcinoma epithelial cell) cells labeling S100 alpha 2 using ab109494. The cells were fixed with 100% Methanol then permeabilized with 0.1% Triton X-100. The cells were then incubated with ab109494 at 1:100 dilution followed by a further incubation with a Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 2 μg/ml (shown in green). Nuclear DNA was labelled in blue with DAPI. Cells were counterstained using ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) at 1:200 dilution (shown in red). Secondary antibody only control: PBS instead of the primary antibody.
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This data was developed using ab109494, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded sections human skin tissue labelling S100 alpha 2 with ab109494 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human skin tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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This data was developed using ab109494, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of Paraffin-embedded sections human lung carcinoma tissue labelling S100 alpha 2 with ab109494 at 1/1000 dilution, followed by a ready to use secondary Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Staining on human lung carcinoma tissue is observed. Counter stained with Haematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0).
The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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All lanes : Anti-S100 alpha 2/S100A2 antibody [EPR5392] (ab109494) at 1/1000 dilution
Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysate at 20 µg
Lane 2 : HaCaT (Human skin keratinocyte) whole cell lysate at 15 µg
Lane 3 : BxPC-3 (Human pancreas adenocarcinoma epithelial cell) whole cell lysate at 15 µg
Lane 4 : A431 (Human epidermoid carcinoma epithelial cell) whole cell lysate at 15 µg
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 11 kDa
Observed band size: 11 kDaThis data was developed using ab109494, the same antibody clone in a different buffer formulation.
Blocking and diluting buffer and concentration: 5% NFDM/TBST.
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This data was developed using ab109494, the same antibody clone in a different buffer formulation.
S100 alpha 2/S100A2 was immunoprecipitated from 0.35 mg HaCaT (Human skin keratinocyte) whole cell lysate 10 µg with ab109494 at 1/20 dilution (1µg). VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.Lane 1: HaCaT (Human skin keratinocyte) whole cell lysate 10 µg
Lane 2: abab109494 IP in HaCaT whole cell lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109494 in HaCaT whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab247877 は論文での使用が確認できていません。