S. cerevisiae RNA-dependent RNA polymerase (RDRP) peptide (ab19119)
Key features and details
- Purity: > 90% HPLC
- Suitable for: Blocking
製品の詳細
-
製品名
S. cerevisiae RNA-dependent RNA polymerase (RDRP) peptide
RNA-dependent RNA polymerase (RDRP) タンパク質・ペプチド 製品一覧 -
精製度
> 90 % HPLC. -
Animal free
No -
由来
Synthetic -
-
生物種
Saccharomyces cerevisiae
-
-
製品の詳細
S. cerevisiae RNA-dependent RNA polymerase (RDRP) peptide
特性
Our Abpromise guarantee covers the use of ab19119 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
-
アプリケーション
Blocking
-
製品の状態
Liquid -
備考
- First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
- If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
- Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
- Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
- Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use. -
Concentration information loading...
前処理および保存
-
保存方法および安定性
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Information available upon request.
関連情報
-
別名
- Protein rdp 1
- Protein rdp1
- Rdp 1
see all -
関連性
RdRP plays a role in the RNA interference (RNAi) pathway. RNAi is the process of sequence-specific,post-transcriptional gene silencing initiated by double-stranded RNA (dsRNA). Recent studies showed small interfering RNAs (siRNA) generated by Dicer from longer dsRNA specifically suppressed expression of genes in somatic and embryo cells. An RdRP activity might provide amplification by replication of long trigger dsRNAs or copying of short siRNAs in a primer-independent manner. The siRNA primed RdRP reaction converts target mRNA into dsRNA, as well as possibly replicating trigger dsRNA. Both products then serve as DICER substrates, initiating the RdRP chain reaction. RNAi is important for heterochromatin formation and accurate chromosome segregation.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab19119 は論文での使用が確認できていません。
