Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Antigen retrieval step
Enzymatic
Blocking step
Serum as blocking agent for 2 hour(s) and 0 minute(s) · Concentration: 10%
Other product details
Dilution
1/100
Incubation time
40 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: biotinylated anti mouse
Host species: Mouse
Clonality: Polyclonal
Conjugation: Biotin
Host species: Mouse
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Antibody: Ryanodine receptor 2, ab 2868, clone 34C. I used this antibody to formalin-fixed and paraffin-embedded human heart sections. It works very nice. The antigen retrieval method is tyrpsine treatment for 40 min at 37 C, the antibody is diluted 1:100 and incubated with the tissue for 48 h at 4C. The distributions profile of Ryanodine in human heart section is punctuate.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Nasrin Perskvist
Verified customer
投稿 Apr 25 2006