Anti-RUNX2 抗体 (ab23981)
Key features and details
- Rabbit polyclonal to RUNX2
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RUNX2 antibody
RUNX2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RUNX2 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Dog
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免疫原
Synthetic peptide corresponding to Human RUNX2 aa 300-400 conjugated to keyhole limpet haemocyanin.
(Peptide available asab26346) -
ポジティブ･コントロール
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab23981の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB | (3) |
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa).
The use of whole cell lysates and milk as a blocking agent will diminish this antibodies signal. Nuclear lysates are recommended. |
| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 57 kDa). The use of whole cell lysates and milk as a blocking agent will diminish this antibodies signal. Nuclear lysates are recommended. |
ターゲット情報
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機能
Transcription factor involved in osteoblastic differentiation and skeletal morphogenesis. Essential for the maturation of osteoblasts and both intramembranous and endochondral ossification. CBF binds to the core site, 5'-PYGPYGGT-3', of a number of enhancers and promoters, including murine leukemia virus, polyomavirus enhancer, T-cell receptor enhancers, osteocalcin, osteopontin, bone sialoprotein, alpha 1(I) collagen, LCK, IL-3 and GM-CSF promoters (By similarity). Inhibits MYST4-dependent transcriptional activation. -
組織特異性
Specifically expressed in osteoblasts. -
関連疾患
Defects in RUNX2 are the cause of cleidocranial dysplasia (CLCD) [MIM:119600]; also known as cleidocranial dysostosis (CCD). CLCD is an autosomal dominant skeletal disorder with high penetrance and variable expressivity. It is due to defective endochondral and intramembranous bone formation. Typical features include hypoplasia/aplasia of clavicles, patent fontanelles, wormian bones (additional cranial plates caused by abnormal ossification of the calvaria), supernumerary teeth, short stature, and other skeletal changes. In some cases defects in RUNX2 are exclusively associated with dental anomalies. -
配列類似性
Contains 1 Runt domain. -
ドメイン
A proline/serine/threonine rich region at the C-terminus is necessary for transcriptional activation of target genes and contains the phosphorylation sites. -
翻訳後修飾
Phosphorylated; probably by MAP kinases (MAPK) (By similarity). Isoform 3 is phosphorylated on Ser-340. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 449531 Dog
- Entrez Gene: 860 Human
- Entrez Gene: 12393 Mouse
- Entrez Gene: 367218 Rat
- Omim: 600211 Human
- SwissProt: Q13950 Human
- SwissProt: Q08775 Mouse
- SwissProt: Q9Z2J9 Rat
see all -
別名
- Acute myeloid leukemia 3 protein antibody
- Alpha subunit 1 antibody
- AML3 antibody
see all
画像
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Western blot - Anti-RUNX2 antibody (ab23981)All lanes : Anti-RUNX2 antibody (ab23981) at 1/1000 dilution
Lane 1 : Bone tissue (tibia) from 17 weeks old Wistar male rats treated with doxorubicin hydrochloride injections for 7 weeks.
Lane 2 : Bone tissue (tibia) from 20 weeks old Wistar female rats.
Lane 3 : Bone tissue (tibia) from 56 weeks old ooforectomized Wistar female rats.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/2000 dilution
Performed under reducing conditions.
Predicted band size: 57 kDa
Observed band size: 57 kDa
Exposure time: 40 seconds
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Western blot - Anti-RUNX2 antibody (ab23981)Anti-RUNX2 antibody (ab23981) at 1 µg/ml + Saos 2 (Human epithelial-like osteosarcoma cell line) Nuclear Extract at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 57 kDa
Exposure time: 8 minutes
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (260)
ab23981 は 260 報の論文で使用されています。
- Zhang D et al. CircRNA-vgll3 promotes osteogenic differentiation of adipose-derived mesenchymal stem cells via modulating miRNA-dependent integrin a5 expression. Cell Death Differ 28:283-302 (2021). PubMed: 32814879
- Chen JY et al. The influence of substrate stiffness on osteogenesis of vascular smooth muscle cells. Colloids Surf B Biointerfaces 197:111388 (2021). PubMed: 33096417
- Long C et al. FOXO3 is targeted by miR-223-3p and promotes osteogenic differentiation of bone marrow mesenchymal stem cells by enhancing autophagy. Hum Cell 34:14-27 (2021). PubMed: 32920731
- Zhang HT et al. TRIM21-regulated Annexin A2 plasma membrane trafficking facilitates osteosarcoma cell differentiation through the TFEB-mediated autophagy. Cell Death Dis 12:21 (2021). PubMed: 33414451
- Jiao D et al. Bidirectional differentiation of BMSCs induced by a biomimetic procallus based on a gelatin-reduced graphene oxide reinforced hydrogel for rapid bone regeneration. Bioact Mater 6:2011-2028 (2021). PubMed: 33426373