Anti-RSPO3 抗体 (ab233113)
Key features and details
- Rabbit polyclonal to RSPO3
- Suitable for: WB, IHC-P
- Reacts with: Human, Pig
- Isotype: IgG
製品の概要
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製品名
Anti-RSPO3 antibody
RSPO3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RSPO3 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Human, Pig
交差が予測される動物種: Cow -
免疫原
Recombinant full length protein (His-T7-tag) corresponding to Human RSPO3 aa 1 to the C-terminus. (Expressed in E.coli).
Database link: Q9BXY4 -
ポジティブ・コントロール
- IHC-P: Human breast cancer, pancreatic cancer, glioma, liver and bile duct cancer tissues. WB: Pig small intestine lysate; Recombinant human RSPO3 protein.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.011% Proclin 300
Constituents: 55.77% Glycerol (glycerin, glycerine), 44.219% PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab233113 was purified by antigen-specific affinity chromatography followed by Protein A affinity chromatography. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab233113の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 31 kDa.
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IHC-P |
Use a concentration of 5 - 20 µg/ml.
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特記事項 |
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WB
Use a concentration of 0.2 - 2 µg/ml. Predicted molecular weight: 31 kDa. |
IHC-P
Use a concentration of 5 - 20 µg/ml. |
ターゲット情報
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機能
Activator of the beta-catenin signaling cascade, leading to TCF-dependent gene activation. Acts both in the canonical Wnt/beta-catenin-dependent pathway, possibly via a direct interaction with Wnt proteins, and in a Wnt-independent beta catenin pathway through a receptor signaling pathway that may not use frizzled/LRP receptors. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. -
組織特異性
Ubiquitously expressed. Expressed at higher level in placenta, small intestine, fetal thymus and lymph node. -
配列類似性
Belongs to the R-spondin family.
Contains 2 FU (furin-like) repeats.
Contains 1 TSP type-1 domain. -
ドメイン
The FU repeats are required for activation and stabilization of beta-catenin. -
細胞内局在
Secreted. - Information by UniProt
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参照データベース
- Entrez Gene: 534650 Cow
- Entrez Gene: 84870 Human
- Omim: 610574 Human
- SwissProt: Q1RMU1 Cow
- SwissProt: Q9BXY4 Human
- Unigene: 135254 Human
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別名
- CRISTIN1 antibody
- hPWTSR antibody
- hRspo3 antibody
see all
画像
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Paraffin-embedded human breast cancer tissue stained for RSPO3 using ab233113 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human pancreatic cancer tissue stained for RSPO3 using ab233113 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human glioma tissue stained for RSPO3 using ab233113 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human liver tissue stained for RSPO3 using ab233113 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Paraffin-embedded human bile duct cancer tissue stained for RSPO3 using ab233113 at 20 µg/ml in immunohistochemical analysis. DAB staining.
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Anti-RSPO3 antibody (ab233113) at 2 µg/ml + Recombinant human RSPO3 protein
Predicted band size: 31 kDa -
Anti-RSPO3 antibody (ab233113) at 2 µg/ml + Pig small intestine lysate
Predicted band size: 31 kDa
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab233113 は 1 報の論文で使用されています。
- Gu H et al. RSPO3 is a marker candidate for predicting tumor aggressiveness in ovarian cancer. Ann Transl Med 8:1351 (2020). PubMed: 33313096