Anti-RPS27/MPS1 抗体 (ab4385)
Key features and details
- Goat polyclonal to RPS27/MPS1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RPS27/MPS1 antibody
RPS27/MPS1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RPS27/MPS1 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat
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免疫原
Synthetic peptide corresponding to Human RPS27/MPS1 aa 2-14 (N terminal).
Sequence:PLAKDLLHPSPEE-C
(Peptide available asab23033) -
特記事項
Official Gene Symbol - RPS27. LocusLink ID - 6232 (human); 57294 (mouse); 94266 (rat). Gene Ontology terms - structural constituent of ribosome; RNA binding; zinc ion binding; protein biosynthesis; cell proliferation; signal transduction; ribosome; cytosolic small ribosomal subunit (sensu Eukarya); intracellular.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% Tris, 0.5% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab4385の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).
1 hour primary incubation is recommended for this product. |
| 特記事項 |
|---|
|
WB
Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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組織特異性
Expressed in a wide variety of actively proliferating cells and tumor tissues. -
配列類似性
Belongs to the ribosomal protein S27e family. - Information by UniProt
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参照データベース
- Entrez Gene: 6232 Human
- Entrez Gene: 100043813 Mouse
- Entrez Gene: 57294 Mouse
- Entrez Gene: 94266 Rat
- Omim: 603702 Human
- SwissProt: P42677 Human
- SwissProt: Q6ZWU9 Mouse
- SwissProt: Q71TY3 Rat
see all -
別名
- 2610206D02Rik antibody
- 3200001M24Rik antibody
- 40S ribosomal protein S27 antibody
see all
画像
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Western blot - Anti-RPS27/MPS1 antibody (ab4385)Anti-RPS27/MPS1 antibody (ab4385) at 0.05 µg/ml + Jurkat lysate (35µg protein in RIPA buffer).
Predicted band size: 10 kDa
Observed band size: 12 kDa why is the actual band size different from the predicted?
Primary incubation was 1 hour. Detected by chemiluminescence.
データシートおよび資料
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Datasheet download
参考文献 (3)
ab4385 は 3 報の論文で使用されています。
- Floristán A et al. Functional analysis of RPS27 mutations and expression in melanoma. Pigment Cell Melanoma Res 33:466-479 (2020). PubMed: 31663663
- Yang ZY et al. Metallopanstimulin-1 regulates invasion and migration of gastric cancer cells partially through integrin ß4. Carcinogenesis N/A:N/A (2013). PubMed: 23803695
- Shin HS et al. Arginine methylation of ribosomal protein S3 affects ribosome assembly. Biochem Biophys Res Commun 385:273-8 (2009). WB ; Human . PubMed: 19460357
