Anti-RPS27/MPS1 抗体 (ab4385)

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Western blot - Anti-RPS27/MPS1 antibody (ab4385)

    Key features and details

    • Goat polyclonal to RPS27/MPS1
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-RPS27/MPS1 antibody
      RPS27/MPS1 一次抗体 製品一覧

    • 製品の詳細

      Goat polyclonal to RPS27/MPS1

    • 由来種

      Goat

    • アプリケーション

      適用あり: WBmore details

    • 種交差性

      交差種: Human
      交差が予測される動物種: Mouse, Rat

    • 免疫原

      Synthetic peptide corresponding to Human RPS27/MPS1 aa 2-14 (N terminal).
      Sequence:

      PLAKDLLHPSPEE-C


      (Peptide available as ab23033)
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 特記事項

      Official Gene Symbol - RPS27. LocusLink ID - 6232 (human); 57294 (mouse); 94266 (rat). Gene Ontology terms - structural constituent of ribosome; RNA binding; zinc ion binding; protein biosynthesis; cell proliferation; signal transduction; ribosome; cytosolic small ribosomal subunit (sensu Eukarya); intracellular.

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    製品の特性

    • 製品の状態

      Liquid

    • 保存方法

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

    • バッファー

      pH: 7.30
      Preservative: 0.02% Sodium azide
      Constituents: 0.5% Tris, 0.5% BSA
    • Concentration information loading...

    • 精製度

      Immunogen affinity purified

    • 特記事項（精製）

      Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

    • ポリ/モノ

      ポリクローナル

    • アイソタイプ

      IgG

    • 研究分野

    アプリケーション

    The Abpromise guarantee

    Abpromise保証は、 次のテスト済みアプリケーションにおけるab4385の使用に適用されます

    アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

    アプリケーション Abreviews 特記事項
    WB
    特記事項
  • 追加情報
    Peptide ELISA: Antibody detection limit dilution 1:64,000.
    WB: Use at a concentration of 0.05 - 0.2 µg/ml. Detects a band of approximately 12 kDa (predicted molecular weight: 10 kDa).


    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.

    • ターゲット情報

    画像

    • Western blot - Anti-RPS27/MPS1 antibody (ab4385)
      Western blot - Anti-RPS27/MPS1 antibody (ab4385)
      Anti-RPS27/MPS1 antibody (ab4385) at 0.05 µg/ml + Jurkat lysate (35µg protein in RIPA buffer).

      Predicted band size: 10 kDa
      Observed band size: 12 kDa why is the actual band size different from the predicted?



      Primary incubation was 1 hour. Detected by chemiluminescence.

    参考文献 (2)

    ab4385 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab4385 は 2 報の論文で使用されています。

    • Yang ZY  et al. Metallopanstimulin-1 regulates invasion and migration of gastric cancer cells partially through integrin ß4. Carcinogenesis N/A:N/A (2013). PubMed: 23803695
    • Shin HS  et al. Arginine methylation of ribosomal protein S3 affects ribosome assembly. Biochem Biophys Res Commun 385:273-8 (2009). WB ; Human . PubMed: 19460357

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