Anti-RPB7 抗体 (ab93352)
Key features and details
- Goat polyclonal to RPB7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RPB7 antibody -
製品の詳細
Goat polyclonal to RPB7 -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Sheep, Cow, Zebrafish -
免疫原
Synthetic peptide corresponding to Human RPB7 aa 118-129 (internal sequence).
Sequence:EFDPNSNPPCYK
Database link: NP_002687.1 -
ポジティブ・コントロール
- Human brain cerebellum lysate
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, Tris buffered saline -
Concentration information loading...
-
精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab93352の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 19 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
---|
WB
Use a concentration of 1 - 3 µg/ml. Detects a band of approximately 21 kDa (predicted molecular weight: 19 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
-
機能
DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB7 is part of a subcomplex with RPB4 that binds to a pocket formed by RPB1, RPB2 and RPB6 at the base of the clamp element. The RBP4-RPB7 subcomplex seems to lock the clamp via RPB7 in the closed conformation thus preventing double stranded DNA to enter the active site cleft. The RPB4-RPB7 subcomplex binds single-stranded DNA and RNA (By similarity). Binds RNA. -
配列類似性
Belongs to the eukaryotic RPB7/RPC8 RNA polymerase subunit family. -
細胞内局在
Nucleus. - Information by UniProt
-
参照データベース
- Entrez Gene: 526320 Cow
- Entrez Gene: 5436 Human
- Entrez Gene: 67710 Mouse
- Entrez Gene: 117017 Rat
- Entrez Gene: 324088 Zebrafish
- Omim: 602013 Human
- SwissProt: Q5E9B8 Cow
- SwissProt: P62487 Human
see all -
別名
- DNA directed RNA polymerase II 19 kDa polypeptide antibody
- DNA directed RNA polymerase II subunit G antibody
- DNA directed RNA polymerase II subunit RPB7 antibody
see all
画像
-
Anti-RPB7 antibody (ab93352) at 3 µg/ml + Human brain cerebellum lysate
Predicted band size: 19 kDa
Observed band size: 21 kDa why is the actual band size different from the predicted?
データシートおよび資料
-
Datasheet download
参考文献 (0)
ab93352 は論文での使用が確認できていません。