Anti-RPA70 抗体 [8C3-D12-H10] (ab176467)
Key features and details
- Mouse monoclonal [8C3-D12-H10] to RPA70
- Suitable for: ICC/IF, IP, WB
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG2a
製品の概要
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製品名
Anti-RPA70 antibody [8C3-D12-H10]
RPA70 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [8C3-D12-H10] to RPA70 -
由来種
Mouse -
アプリケーション
適用あり: ICC/IF, IP, WBmore details -
種交差性
交差種: Mouse, Rat, Human, African green monkey -
免疫原
Recombinant fragment corresponding to Human RPA70. Purified recombinant human RPA70 protein fragments expressed in E.coli
Database link: P27694 -
ポジティブ・コントロール
- COS7, HT1080, A431, MCF7, K562, HeLa, 293T, 3T3 and C6 cell lysate
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特記事項
This product was changed from ascites to tissue culture supernatant on 17.08.2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.03% Proclin 300
Constituent: 50% Glycerol -
Concentration information loading...
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精製度
Concentrated Culture Supernatant -
ポリ/モノ
モノクローナル -
クローン名
8C3-D12-H10 -
アイソタイプ
IgG2a -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab176467の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ICC/IF | (1) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB | (1) |
1/1000. Predicted molecular weight: 68 kDa.
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特記事項 |
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ICC/IF
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
1/1000. Predicted molecular weight: 68 kDa. |
ターゲット情報
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機能
Plays an essential role in several cellular processes in DNA metabolism including replication, recombination and DNA repair. Binds and subsequently stabilizes single-stranded DNA intermediates and thus prevents complementary DNA from reannealing.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
配列類似性
Belongs to the replication factor A protein 1 family. -
翻訳後修飾
Phosphorylated upon DNA damage, probably by ATM or ATR.
Sumoylated on lysine residues Lys-449 and Lys-577, with Lys-449 being the major site. Sumoylation promotes recruitment of RAD51 to the DNA damage foci to initiate DNA repair through homologous recombinaison. Desumoylated by SENP6. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6117 Human
- Entrez Gene: 68275 Mouse
- Entrez Gene: 287524 Rat
- Omim: 179835 Human
- SwissProt: P27694 Human
- SwissProt: Q8VEE4 Mouse
- Unigene: 461925 Human
- Unigene: 180734 Mouse
see all -
別名
- Dmrpa1 antibody
- Drosophila Replication Protein A antibody
- DRPA antibody
see all
画像
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution
Lane 1 : C6 Cell lysate
Lane 2 : COS7 Cell lysate
Lane 3 : K562 Cell lysate
Lane 4 : HeLa Cell lysate
Predicted band size: 68 kDa -
Immunocytochemistry/Immunofluorscence analysis of HeLa cells fixed with paraformaldehyde, labeling RPA70 with ab176467 at 1/100. Left - RPA70, Middle - nuclear staining, Right - Merge. This image was produced with antibody produced in ascites.
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467) at 1/1000 dilution
Lane 1 : 293T cell lysate
Lane 2 : 3T3 cell lysate
Lane 3 : K562 cell lysate
Lane 4 : HeLa cell lysate
Lane 5 : C6 cell lysate
Predicted band size: 68 kDaThis image was produced with antibody produced in ascites.
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All lanes : Anti-RPA70 antibody [8C3-D12-H10] (ab176467)
Lane 1 : COS7 cell lysate
Lane 2 : HT1080 cell lysate
Lane 3 : A431 cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : K562 cell lysate
Lane 6 : HeLa cell lysate
Predicted band size: 68 kDaThis image was produced with antibody produced in ascites.
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Immunoprecipitation analysis of HeLa cell lysate labeling RPA70 with ab176467. This image was produced with antibody produced in ascites.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (4)
ab176467 は 4 報の論文で使用されています。
- Hu C et al. Beta human papillomavirus 8 E6 allows colocalization of non-homologous end joining and homologous recombination repair factors. PLoS Pathog 18:e1010275 (2022). PubMed: 35148356
- Kang JW et al. PUMA facilitates EMI1-promoted cytoplasmic Rad51 ubiquitination and inhibits DNA repair in stem and progenitor cells. Signal Transduct Target Ther 6:129 (2021). PubMed: 33785736
- Fowler S et al. LIM Protein Ajuba associates with the RPA complex through direct cell cycle-dependent interaction with the RPA70 subunit. Sci Rep 8:9536 (2018). PubMed: 29934626
- Ghospurkar PL et al. Phosphorylation and cellular function of the human Rpa2 N-terminus in the budding yeast Saccharomyces cerevisiae. Exp Cell Res 331:183-99 (2015). WB . PubMed: 25499885