Anti-RPA32/RPA2 (phospho S4 + S8) 抗体 (ab87277)


  • 製品名
    Anti-RPA32/RPA2 (phospho S4 + S8) antibody
    RPA32/RPA2 一次抗体 製品一覧
  • 製品の詳細
    Rabbit polyclonal to RPA32/RPA2 (phospho S4 + S8)
  • 由来種
  • アプリケーション
    適用あり: IHC-P, ICC/IFmore details
  • 種交差性
    交差種: Mouse, Human
    交差が予測される動物種: Rhesus monkey, Gorilla, Orangutan, Bat
  • 免疫原

    A dually phosphorylated synthetic peptide, which represented a portion of Human RPA32/RPA2, surrounding phosphorylated serines that corresponded to positions 4 and 8 (NP_002937.1, GeneID 6118).

  • ポジティブ・コントロール
    • Human Controls: Breast Carcinoma, Colon Carcinoma, Ovarian Carcinoma, Prostate Carcinoma, Stomach Adenocarcinoma, Testicular Seminoma
      Mouse Controls: Colon Carcinoma CT26
  • 特記事項

    Concentration is optimized for IHC and not determined



Our Abpromise guarantee covers the use of ab87277 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/100 - 1/500. Epitope exposure with tris-EDTA pH9.0 buffer will enhance staining.
ICC/IF 1/100 - 1/500.


  • 機能
    Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
    Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
  • 翻訳後修飾
    Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
  • 細胞内局在
    Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 60S acidic ribosomal protein P1 antibody
    • AA409079 antibody
    • AI325195 antibody
    • AU020965 antibody
    • ik:tdsubc_2g1 antibody
    • M(2)21C antibody
    • MGC137236 antibody
    • OTTHUMP00000004008 antibody
    • p32 antibody
    • p34 antibody
    • RCJMB04_6d17 replication protein A2, 32kDa antibody
    • REPA2 antibody
    • Replication factor A protein 2 antibody
    • Replication protein A 32 kDa subunit antibody
    • Replication protein A 32kDa subunit antibody
    • Replication protein A 34 kDa subunit antibody
    • Replication protein A antibody
    • Replication Protein A2 (32kDa) antibody
    • Replication protein A2 antibody
    • Replication protein A2, 32kDa antibody
    • RF-A protein 2 antibody
    • Rf-A2 antibody
    • RFA antibody
    • RFA2_HUMAN antibody
    • RP-A p32 antibody
    • RP-A p34 antibody
    • RP21C antibody
    • RPA 2 antibody
    • RPA 32 antibody
    • RPA antibody
    • Rpa2 antibody
    • RPA32 antibody
    • RPA34 antibody
    • RpLP1 antibody
    • RpP2 antibody
    • xx:tdsubc_2g1 antibody
    • zgc:109822 antibody
    see all


  • Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/250 dilution, with mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right). Detection: DAB staining.
  • Immunohistochemistry analysis of Mouse colon carcinoma using ab87277 at 1/250 dilution, with mock phosphatase treated section (left) or calf intestinal phosphatase treated section (right). Detection: DAB staining.
  • Immunohistochemistry analysis of Human breast carcinoma using ab87277 at 1/100 dilution. Detection: Red-fluorescent goat anti-rabbit IgG at 1/100 dilution.


This product has been referenced in:
  • Cottineau J  et al. Inherited GINS1 deficiency underlies growth retardation along with neutropenia and NK cell deficiency. J Clin Invest 127:1991-2006 (2017). Read more (PubMed: 28414293) »
  • Kanu N  et al. DNA replication stress mediates APOBEC3 family mutagenesis in breast cancer. Genome Biol 17:185 (2016). WB ; Human . Read more (PubMed: 27634334) »

See all 3 Publications for this product

レビューと Q&A

DISCOUNT CODE: *** Expiration date: March 29, 2012 I am very pleased to hear you would like to test ab87277 in Western blot. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for...

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