Anti-RPA32/RPA2 (phospho S33) 抗体 (ab211877)
Key features and details
- Rabbit polyclonal to RPA32/RPA2 (phospho S33)
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
-
製品名
Anti-RPA32/RPA2 (phospho S33) antibody
RPA32/RPA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA32/RPA2 (phospho S33) -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chimpanzee, Rhesus monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human RPA32/RPA2 (phospho S33). (NP_002937.1)
Database link: P15927 -
ポジティブ・コントロール
- HeLa whole cell lysate treated with 100 µM etoposide for 4 hours.
-
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: 99% Tris citrate/phosphate
pH: 7 to 8 -
Concentration information loading...
-
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
-
Compatible Secondaries
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab211877の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/5000. Predicted molecular weight: 29 kDa.
Numerous bands representing proteins that migrate more slowly than 60 to 70 kDa have been observed during WB. Under some conditions, at least two of these may be more intense than the band that represents phospho RPA32/RPA2 (S33). |
特記事項 |
---|
WB
1/1000 - 1/5000. Predicted molecular weight: 29 kDa. Numerous bands representing proteins that migrate more slowly than 60 to 70 kDa have been observed during WB. Under some conditions, at least two of these may be more intense than the band that represents phospho RPA32/RPA2 (S33). |
ターゲット情報
-
機能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻訳後修飾
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
細胞内局在
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
-
参照データベース
- Entrez Gene: 456680 Chimpanzee
- Entrez Gene: 101144875 Gorilla
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 100172265 Orangutan
- Entrez Gene: 716612 Rhesus monkey
- Omim: 179836 Human
- SwissProt: P15927 Human
see all -
別名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
画像
-
All lanes : Anti-RPA32/RPA2 (phospho S33) antibody (ab211877) at 0.4 µg/ml
Lane 1 : HeLa whole cell lysate treated with 100 µM etoposide for 4 hours
Lane 2 : HeLa whole cell lysate mock treated
Lysates/proteins at 50 µg per lane.
Developed using the ECL technique.
Predicted band size: 29 kDa
Exposure time: 10 secondsLower panel shows Western Blotting for total RPA32/RPA2 using an affinity purified rabbit anti-RPA32/RPA2 antibody at 0.1 µg/ml with an exposure time of 10 seconds.
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (7)
ab211877 は 7 報の論文で使用されています。
- Berger ND et al. High replication stress and limited Rad51-mediated DNA repair capacity, but not oxidative stress, underlie oligodendrocyte precursor cell radiosensitivity. NAR Cancer 4:zcac012 (2022). PubMed: 35425901
- Hussain M et al. A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism. iScience 25:104591 (2022). PubMed: 35789855
- Xu T et al. A small molecule inhibitor of the UBE2F-CRL5 axis induces apoptosis and radiosensitization in lung cancer. Signal Transduct Target Ther 7:354 (2022). PubMed: 36253371
- Amato R et al. G-quadruplex Stabilization Fuels the ALT Pathway in ALT-positive Osteosarcoma Cells. Genes (Basel) 11:N/A (2020). PubMed: 32183119
- Edwards DS et al. BRD4 Prevents R-Loop Formation and Transcription-Replication Conflicts by Ensuring Efficient Transcription Elongation. Cell Rep 32:108166 (2020). PubMed: 32966794
- Shi Y et al. Phosphorylation of seryl-tRNA synthetase by ATM/ATR is essential for hypoxia-induced angiogenesis. PLoS Biol 18:e3000991 (2020). PubMed: 33351793
- Bélanger F et al. Replication Protein A Availability during DNA Replication Stress Is a Major Determinant of Cisplatin Resistance in Ovarian Cancer Cells. Cancer Res 78:5561-5573 (2018). PubMed: 30072396