Name: Anti-RPA32/RPA2 antibody [EPR2876(2)]
Brand: RabMAb
SKU: ab109084
Rating: 3 (1 reviews)

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Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2876(2)] to RPA32/RPA2
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
Reacts with: Rat, Human

Carrier Free

製品名

Anti-RPA32/RPA2 antibody [EPR2876(2)]
RPA32/RPA2 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR2876(2)] to RPA32/RPA2
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details
適用なし: IP
種交差性

交差種: Rat, Human
免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
ポジティブ･コントロール
- WB: HeLa, HUVEC, HepG2, and MCF7 cell lysates; IHC-P: Human colon and Human ovary carcinoma tissues. ICC/IF: C6 and HeLa cells Flow Cyt (intra): C6 and HeLa cells
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
バッファー

pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
Concentration information loading...
精製度

Tissue culture supernatant
ポリ/モノ

モノクローナル
クローン名

EPR2876(2)
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-RPA32/RPA2 antibody [EPR2876(2)] - BSA and Azide free (ab247754)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- Hep G2 nuclear extract lysate (ab14660)
- MCF7 nuclear extract lysate (ab14860)
Recombinant Protein
- Recombinant Human RPA32/RPA2 protein (ab101216)

The Abpromise guarantee

Abpromise保証は、次のテスト済みアプリケーションにおけるab109084の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション	Abreviews	特記事項
Flow Cyt (Intra)		Use at an assay dependent concentration.
WB		1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
ICC/IF	(1)	Use at an assay dependent concentration.

特記事項
Flow Cyt (Intra) Use at an assay dependent concentration.
WB 1/1000 - 1/10000. Predicted molecular weight: 29 kDa.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Antigen retrieval is recommended.
ICC/IF Use at an assay dependent concentration.

追加情報

Is unsuitable for IP.

機能

Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange.
翻訳後修飾

Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1.
細胞内局在

Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage.
Target information above from: UniProt accession P15927 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 6118 Human
- Entrez Gene: 59102 Rat
- Omim: 179836 Human
- SwissProt: P15927 Human
- SwissProt: Q63528 Rat
- Unigene: 703070 Human
- Unigene: 79411 Human
- Unigene: 40389 Rat
別名
- 60S acidic ribosomal protein P1 antibody
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- M(2)21C antibody
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- RCJMB04_6d17 replication protein A2, 32kDa antibody
- REPA2 antibody
- Replication factor A protein 2 antibody
- Replication protein A 32 kDa subunit antibody
- Replication protein A 32kDa subunit antibody
- Replication protein A 34 kDa subunit antibody
- Replication protein A antibody
- Replication Protein A2 (32kDa) antibody
- Replication protein A2 antibody
- Replication protein A2, 32kDa antibody
- RF-A protein 2 antibody
- Rf-A2 antibody
- RFA antibody
- RFA2_HUMAN antibody
- RP-A p32 antibody
- RP-A p34 antibody
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- RPA antibody
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- RPA32 antibody
- RPA34 antibody
- RpLP1 antibody
- RpP2 antibody
- xx:tdsubc_2g1 antibody
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see all

Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Immunocytochemistry/ Immunofluorescence analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1:50 (9.34 µg/ml). Cells were fixed in 100% Methanol and permeabilized with None. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Intracellular Flow Cytometry analysis of C6 (rat glial tumor glial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1/460 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
Flow Cytometry (Intracellular) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma epithelial cell) cells labeling RPA32/RPA2 with purified ab109084 at 1/460 dilution (1.01 µg/ml) - Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor^® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) - Black. Unlabeled control - Blue. Untreated cells - Green
Western blot - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

All lanes : Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084) at 1/1000 dilution

Lane 1 : HeLa cell lysate
Lane 2 : HUVEC cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 29 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

ab109084 at 1/250 dilution staining RPA32/RPA2 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

ab109084 at 1/250 dilution staining RPA32/RPA2 in Human ovary carcinoma by Immunohistochemistry, Paraffin-embedded tissue.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-RPA32/RPA2 antibody [EPR2876(2)] (ab109084)

Western blot protocols
Immunohistochemistry protocols

Click here to view the general protocols

Datasheet download

Download

ab109084 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab109084 は 1 報の論文で使用されています。

Matos DA et al. ATR Protects the Genome against R Loops through a MUS81-Triggered Feedback Loop. Mol Cell 77:514-527.e4 (2020). PubMed: 31708417

レビューと Q&A

Show All レビュー Q&A

レビューを送る質問を送る

1-2 of 2 Abreviews or Q&A

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa cells)

Specification

HeLa cells

Fixative

Formaldehyde

Permeabilization

Yes - 0.2% Triton X100

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: .5% · Temperature: 22°C

The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Aug 30 2012

Question

Customer kindly contacted us to inquire about RPA32/RPA2 antibodies and testing of ab109084 in ICC/IF.

Abcam community

Verified customer

Asked on Jul 23 2012

Answer

I am very pleased to hear you would like to accept our offer and test ab109084 inICC/IF. This code will give you: 1 free PRIMARY ANTIBODY OR VALUE OFF OF ORDER before the expiration date. To redeem this offer, please submit an Abreview for ICC/IF and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Abcam Scientific Support

Answered on Jul 23 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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Key features and details

Related conjugates and formulations

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

The Abpromise guarantee

ターゲット情報

機能

翻訳後修飾

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

Datasheet download

参考文献 (1)

レビューと Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-RPA32/RPA2 antibody [EPR2876(2)]