Anti-RPA32/RPA2 抗体 (ab97594)
Key features and details
- Rabbit polyclonal to RPA32/RPA2
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RPA32/RPA2 antibody
RPA32/RPA2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RPA32/RPA2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Cow
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免疫原
Recombinant protein fragment containing a sequence corresponding to a region within amino acids 1 and 172 of human RPA32/RPA2 (NP_002937)
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ポジティブ･コントロール
- 293T, A431, H1299, HeLa, HepG2, MOLT4 and Raji cell lysates A549 xenograft HeLa cells
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading... -
精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97594の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 29 kDa.
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| IHC-P |
1/100 - 1/250.
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| ICC/IF |
1/100 - 1/200.
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| 特記事項 |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 29 kDa. |
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IHC-P
1/100 - 1/250. |
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ICC/IF
1/100 - 1/200. |
ターゲット情報
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機能
Required for DNA recombination, repair and replication. The activity of RP-A is mediated by single-stranded DNA binding and protein interactions.
Functions as component of the alternative replication protein A complex (aRPA). aRPA binds single-stranded DNA and probably plays a role in DNA repair; it does not support chromosomal DNA replication and cell cycle progression through S-phase. In vitro, aRPA cannot promote efficient priming by DNA polymerase alpha but supports DNA polymerase delta synthesis in the presence of PCNA and replication factor C (RFC), the dual incision/excision reaction of nucleotide excision repair and RAD51-dependent strand exchange. -
翻訳後修飾
Phosphorylated in a cell-cycle-dependent manner (from the S phase until mitosis). Phosphorylated by ATR upon DNA damage, which promotes its translocation to nuclear foci. Can be phosphorylated in vitro by PRKDC/DNA-PK in the presence of Ku and DNA, and by CDK1. -
細胞内局在
Nucleus. Nucleus > PML body. Also present in PML nuclear bodies. Redistributes to discrete nuclear foci upon DNA damage. - Information by UniProt
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参照データベース
- Entrez Gene: 507912 Cow
- Entrez Gene: 6118 Human
- Entrez Gene: 19891 Mouse
- Entrez Gene: 59102 Rat
- Omim: 179836 Human
- SwissProt: P15927 Human
- SwissProt: Q62193 Mouse
- SwissProt: Q63528 Rat
see all -
別名
- 60S acidic ribosomal protein P1 antibody
- AA409079 antibody
- AI325195 antibody
see all
画像
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Western blot - Anti-RPA32/RPA2 antibody (ab97594)All lanes : Anti-RPA32/RPA2 antibody (ab97594) at 1/1000 dilution
Lane 1 : 293T whole cell lysate
Lane 2 : A431 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 29 kDa
Gel conc 12% (SDS PAGE) -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RPA32/RPA2 antibody (ab97594)ab97594, at a 1/100 dilution, staining human RPA32/RPA2 in A549 xenograft, using Immunohistochemistry, Formalin/PFA-fixed paraffin-embedded tissue. -
Immunocytochemistry/ Immunofluorescence - Anti-RPA32/RPA2 antibody (ab97594)Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using ab97594 at a 1/200 dilution (top image) and merged with DNA probe (bottom image).
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (2)
ab97594 は 2 報の論文で使用されています。
- Izumi H & Funa K Telomere Function and the G-Quadruplex Formation are Regulated by hnRNP U. Cells 8:N/A (2019). PubMed: 31035352
- Chan YW et al. Unresolved recombination intermediates lead to ultra-fine anaphase bridges, chromosome breaks and aberrations. Nat Cell Biol 20:92-103 (2018). PubMed: 29255170