Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (skin fibroblasts)
Specification
skin fibroblasts
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X100
Blocking step
Serum as blocking agent for 20 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 30 minute(s) · Temperature: 37°C · Diluent: 5% serum, 1X PBS
Secondary antibody
Name
Non-Abcam antibody was used: Anti-mouse
Host species: Sheep
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Sheep
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/600
Additional data
Additional Notes
Cells were treated with 2mM of hydroxyurea for 16 hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Kok-Lung Chan
Verified customer
投稿 Nov 08 2007