Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Formaldehyde
Permeabilization
Yes - 0.5% Triton X-100
Blocking step
BSA as blocking agent for 15 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/500
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBS 1X+5% BSA+ 0.05% Tween-20
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit polyclonal anti-Mouse
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500
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投稿 Oct 28 2011