Anti-ROR alpha/RORA 抗体 [EPR23719-48] (ab278099)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR23719-48] to ROR alpha/RORA
- Suitable for: IHC-P, IHC-Fr, IP
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
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製品名
Anti-ROR alpha/RORA antibody [EPR23719-48]
ROR alpha/RORA 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR23719-48] to ROR alpha/RORA -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, IHC-Fr, IPmore details
適用なし: WB -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- IHC-Fr: Mouse Cerebellum tissue IHC-P: Mouse Cerebellum tissue; Rat Cerebellum tissue. IP: Mouse cerebellum tissue lysate; Rat cerebellum tissue lysate, SH-SY5Y whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.01% Sodium azide
Constituents: 59.94% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR23719-48 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab278099の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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IHC-Fr |
1/50.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
|
IP |
1/30.
|
特記事項 |
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IHC-P
1/800. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
IHC-Fr
1/50. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) |
IP
1/30. |
ターゲット情報
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機能
Orphan nuclear receptor. Binds DNA as a monomer to hormone response elements (HRE) containing a single core motif half-site preceded by a short A-T-rich sequence. This isomer binds to the consensus sequence 5'-[AT][TA]A[AT][CGT]TAGGTCA-3'. Regulates a number of genes involved in lipid metabolism such as apolipoproteins AI, APOA5, CIII, CYP71 and PPARgamma, in cerebellum and photoreceptor development including PCP2, OPN1SW, OPN1SM AND ARR3, in circadian rhythm with BMAL1, and skeletal muscle development with MYOD1. Possible receptor for cholesterol or one of its derivatives. -
組織特異性
Widely expressed in a number of tissues. -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
翻訳後修飾
Phosphorylation by PKC in neurons inhibits transcriptional activity. Phosphorylated on Thr-216 by ERK2 in vitro.
Sumoylated by SENP1 and SENP2. Sumoylation, promoted by PIAS2, PIAS3, PIASy but not PIAS1, enhances the transcriptional activity. Desumoylated by SENP1.
Ubiquitinated. Ubiquitination is required for efficient transcriptional activity and is prevented by Hairless. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 6095 Human
- Entrez Gene: 19883 Mouse
- Entrez Gene: 300807 Rat
- Omim: 600825 Human
- SwissProt: P35398 Human
- SwissProt: P51448 Mouse
- Unigene: 560343 Human
- Unigene: 655155 Human
see all -
別名
- fhl2a antibody
- NR1F1 antibody
- Nuclear receptor ROR alpha antibody
see all
画像
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ROR alpha/RORA was immunoprecipitated from SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate with ab278099 at 1/30 dilution (2µg in 0.35mg lysates). Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1 (Input): SH-SY5Y (human neuroblastoma epithelial cell), whole cell lysate, 10 μg
Lane 2 (+): SH-SY5Y whole cell lysate
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab278099 in SH-SY5Y whole cell lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 180 seconds
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Immunohistochemical analysis of paraffin-embedded Mouse cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/800 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum ( PMID:14966198). The section was incubated with ab278099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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ROR alpha/RORA was immunoprecipitated from 0.35 mg Mouse cerebellum tissue lysate with ab278099 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Mouse cerebellum tissue lysate 10ug
Lane 2: ab278099 IP in Mouse cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278099 in mouse cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Positive staining on mouse cerebellum is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
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ROR alpha/RORA was immunoprecipitated from 0.35 mg Rat cerebellum tissue lysate with ab278099 at 1/30 dilution. Western blot was performed on the immunoprecipitate using ab278099 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.
Lane 1: Rat cerebellum tissue lysate 10ug
Lane 2: ab278099 IP in Rat cerebellum tissue lysate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab278099 in rat cerebellum tissue lysate
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
Exposure time: 3 minutes
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Immunohistochemical analysis of paraffin-embedded Rat cerebellum tissue labeling ROR alpha/RORA with ab278099 at 1/800 dilution followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on rat cerebellum. The section was incubated with ab278099 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument Counterstained with Hematoxylin.
Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).
Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins
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Immunohistochemical analysis of 4% PFA-fixed, 0.2% Triton X-100 permeabilized frozen Mouse kidney tissue labeling ROR alpha/RORA with ab278099 at 1/50 dilution followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1/1000 dilution (Green). Negative control: no staining on mouse kidney (PMID: 9226375) is observed. The nuclear counterstain was DAPI (Blue).
Secondary antibody control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)at 1/1000 dilution.
Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (1)
ab278099 は 1 報の論文で使用されています。
- Liu CH et al. Genetic deficiency and pharmacological modulation of RORα regulate laser-induced choroidal neovascularization. Aging (Albany NY) 15:37-52 (2023). PubMed: 36626253