Anti-ROMO1 抗体 [OTI2C12] (ab236409)
Key features and details
- Mouse monoclonal [OTI2C12] to ROMO1
- Suitable for: WB, ICC/IF, IHC-P
- Reacts with: Mouse, Dog, Human, African green monkey
- Isotype: IgG1
製品の概要
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製品名
Anti-ROMO1 antibody [OTI2C12] -
製品の詳細
Mouse monoclonal [OTI2C12] to ROMO1 -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-Pmore details -
種交差性
交差種: Mouse, Dog, Human, African green monkey
交差が予測される動物種: Cow, Pig -
免疫原
Recombinant full length protein corresponding to Human ROMO1 aa 1 to the C-terminus. (NP_542786) produced in HEK-293T cells.
Database link: P60602 -
ポジティブ・コントロール
- WB: HepG2, HeLa, SVT2, A549, COS-7, Jurkat and MDCK whole cell lysates; pCMV6-ENTRY ROMO1 transfected HEK-293T whole cell lysate. IHC-P: Human breast, breast adenocarcinoma, colon adenocarcinoma, ovary adenocarcinoma, endometrial adenocarcinoma, normal pancreas, thyroid carcinoma, normal prostate and normal kidney tissue. ICC/IF: COS-7 and HeLa cells.
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特記事項
Clone OTI2C12 (formerly 2C12)
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
OTI2C12 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236409の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Predicted molecular weight: 8 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/150.
Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes |
特記事項 |
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WB
1/2000. Predicted molecular weight: 8 kDa. |
ICC/IF
1/100. |
IHC-P
1/150. Heat-induced epitope retrieval by 10 mM citric buffer, pH 6.0, 100°C for 10 minutes |
ターゲット情報
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機能
Induces production of reactive oxygen species (ROS) which are necessary for cell proliferation. May play a role in inducing oxidative DNA damage and replicative senescence. -
組織特異性
Up-regulated in a number of cancer cell lines when compared to a normal lung fibroblast cell line. -
配列類似性
Belongs to the MGR2 family. -
発生段階
Expression increases in senescent cells. -
細胞内局在
Mitochondrion membrane. - Information by UniProt
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参照データベース
- Entrez Gene: 140823 Human
- Entrez Gene: 67067 Mouse
- SwissProt: Q3SZV8 Cow
- SwissProt: P60602 Human
- SwissProt: P60603 Mouse
- SwissProt: A1XQR6 Pig
- Unigene: 472564 Human
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別名
- bA353C18.2 antibody
- C20orf52 antibody
- Mitochondrial targeting GXXXG protein antibody
see all
画像
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All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY control, whole cell lysate
Lane 2 : HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) transfected with pCMV6-ENTRY ROMO1, whole cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 8 kDa -
All lanes : Anti-ROMO1 antibody [OTI2C12] (ab236409) at 1/2000 dilution
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HeLa (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 3 : SV-T2 (Mouse cell line) whole cell lysate
Lane 4 : A549 (human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 5 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysate
Lane 6 : Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate
Lane 7 : MDCK (canine kidney cell line) whole cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 8 kDa -
Paraffin-embedded human breast tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human breast adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human colon adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human ovary adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human thyroid tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrial adenocarcinoma tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human prostate tissue stained for ROMO1 using ab236409 at 1/150 dilution in immunohistochemical analysis.
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COS-7 (African green monkey kidney fibroblast-like cell line) cells transiently transfected with pCMV6-ENTRY ROMO1 stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.
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HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for ROMO1 (green) using ab236409 at 1/100 dilution in ICC/IF.
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab236409 は論文での使用が確認できていません。