Key features and details
- Rabbit polyclonal to Robo1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
保存方法Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
バッファーPreservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
Concentration information loading...
精製度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab7279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|WB||1/500 - 1/3000. Detects a band of approximately 181 kDa.|
機能Receptor for SLIT1 and SLIT2 which are thought to act as molecular guidance cue in cellular migration, including axonal navigation at the ventral midline of the neural tube and projection of axons to different regions during neuronal development. In axon growth cones, the silencing of the attractive effect of NTN1 by SLIT2 may require the formation of a ROBO1-DCC complex. May be required for lung development.
組織特異性Widely expressed, with exception of kidney.
配列類似性Belongs to the immunoglobulin superfamily. ROBO family.
Contains 3 fibronectin type-III domains.
Contains 5 Ig-like C2-type (immunoglobulin-like) domains.
翻訳後修飾Ubiquitinated. May be deubiquitinated by USP33.
- Information by UniProt
- Deleted in U twenty twenty antibody
- DUTT 1 antibody
- DUTT1 antibody
Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HeLa whole cell lysate at 30 µg
This image shows staining of human brain tissue.
Anti-Robo1 antibody (ab7279) at 1/1000 dilution + HEK 293T cells at 25 µg
Developed using the ECL technique.
Performed under reducing conditions.
ab7279 at a 1/2000 dilution staining Robo1 in mouse muscle satellite cells by Immunohistochemistry/ Immunofluorescence, incubated for 12 hours at +4°C. PFA fixed. Blocked with 5% serum for 1 hour at 20°C. Secondary used at 1/500 monoclonal Goat anti-rabbit conjugated to Alexa Fluor 594 (red).
ab7279 staining Robo1 in undifferentiated, immortalized human podocytes by Immunocytochemistry/ Immunofluorescence.
Cells were fixed with 2% paraformaldehyde and 4% sucrose at room temperature for 10 minutes. The cells were then washed once with PBS, permeabilized with 0.3% Triton X-100 for 10 minutes and incubated with blocking solution (2% FCS, 2% BSA, 0.2% fish gelatin) for 30 minutes, before further incubation with ab7279 for 1 hour. An Alexa Fluor 488 goat anti-rabbit IgG secondary antibody was used at a dilution of 1/200. DAPI was used for nuclear counterstaining.
ab7279 は 38 報の論文で使用されています。
- Yao Y et al. Activation of Slit2/Robo1 Signaling Promotes Tumor Metastasis in Colorectal Carcinoma through Activation of the TGF-ß/Smads Pathway. Cells 8:N/A (2019). PubMed: 31242633
- Xia Y et al. Reduced USP33 expression in gastric cancer decreases inhibitory effects of Slit2-Robo1 signalling on cell migration and EMT. Cell Prolif 52:e12606 (2019). PubMed: 30896071
- Rezniczek GA et al. ROBO1 Expression in Metastasizing Breast and Ovarian Cancer: SLIT2-induced Chemotaxis Requires Heparan Sulfates (Heparin). Anticancer Res 39:1267-1273 (2019). PubMed: 30842157
- Xu R et al. Targeting skeletal endothelium to ameliorate bone loss. Nat Med 24:823-833 (2018). PubMed: 29785024
- Dou C et al. Slit3 regulates migration of endothelial progenitor cells by activation of the RhoA/Rho kinase pathway. Int J Clin Exp Pathol 11:3398-3404 (2018). PubMed: 31949717