Anti-RNF8 抗体 [EPR6311(2)] (ab128872)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR6311(2)] to RNF8
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
製品の概要
-
製品名
Anti-RNF8 antibody [EPR6311(2)]
RNF8 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR6311(2)] to RNF8 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details
適用なし: ICC/IF,IHC-P or IP -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide within Human RNF8 aa 50-150. The exact sequence is proprietary.
-
ポジティブ・コントロール
- WB: Human fetal brain and fetal thymus lysates. Mouse breast and ovary lysates. PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate
-
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant -
Concentration information loading...
-
精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR6311(2) -
アイソタイプ
IgG -
研究分野
関連製品
-
Alternative Versions
-
Isotype control
-
Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab128872の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa).
|
特記事項 |
---|
WB
1/1000 - 1/10000. Detects a band of approximately 60 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
-
機能
E3 ubiquitin-protein ligase required for assembly of repair proteins to sites of DNA damage. Catalyzes the 'Lys-63'-linked ubiquitination of histone H2A and H2AX. Following DNA double-strand breaks (DSBs), it is recruited to the sites of damage by ATM-phosphorylated MDC1, mediates the ubiquitination of histones H2A and H2AX, thereby promoting the formation of TP53BP1 and BRCA1 ionizing radiation-induced foci (IRIF). Promotes the formation of 'Lys-63'-linked polyubiquitin chains and functions with the specific ubiquitin-conjugating UBE2N/UBC13. Substrates that are polyubiquitinated at 'Lys-63' are usually not targeted for degradation. Enforces the G2/M DNA damage checkpoint. Controls the recruitment of UIMC1-BRCC3 (RAP80-BRCC36) and PAXIP1/PTIP to DNA damage sites following DNA double-strand breaks (DSBs). Ubiquitination of histone H2A requires UBE2N but not MMS2 (UBE2V2). May also ubiquitinate histone H2B. Catalyzes the 'Lys-63'-linked ubiquitination of PCNA. May be required for proper exit from mitosis after spindle checkpoint activation and may regulate cytokinesis. May play a role in the regulation of RXRA-mediated transcriptional activity. Not involved in RXRA ubiquitination by UBE2E2. -
組織特異性
Ubiquitous. In fetal tissues, highest expression in brain, thymus and liver. In adult tissues, highest levels in brain and testis, lowest levels in peripheral blood cells. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Belongs to the RNF8 family.
Contains 1 FHA domain.
Contains 1 RING-type zinc finger. -
発生段階
Low levels at the G1-S boundary increase in intensity during S phase and until the end of the G2 phase. Abruptly decreases in late mitosis (at protein level). Barely detectable in anaphase. -
ドメイン
The FHA domain specifically recognizes and binds ATM-phosphorylated MDC1 and Thr-4827 phosphorylated HERC2. -
翻訳後修飾
Autoubiquitinated through 'Lys-48' and 'Lys-63' of ubiquitin. 'Lys-63' polyubiquitination is mediated by UBE2N. 'Lys-29'-type polyubiquitination is also observed, but it doesn't require its own functional RING-type zinc finger. -
細胞内局在
Nucleus. Midbody. Following DNA double-strand breaks, recruited to the sites of damage. During prophase, concomitant with nuclear envelope breakdown, localizes throughout the cell, with a dotted pattern. In telophase, again in the nucleus and also with a discrete dotted pattern in the cytoplasm. In late telophase and during cytokinesis, localizes in the midbody of the tubulin bridge joining the daughter cells. Does not seem to be associated with condensed chromosomes at any time during the cell cycle. - Information by UniProt
-
参照データベース
- Entrez Gene: 9025 Human
- Entrez Gene: 58230 Mouse
- Entrez Gene: 361815 Rat
- Omim: 611685 Human
- SwissProt: O76064 Human
- SwissProt: Q8VC56 Mouse
- SwissProt: Q4KLN8 Rat
- Unigene: 485278 Human
see all -
別名
- C3HC4 type zinc finger protein antibody
- E3 ubiquitin protein ligase RNF8 antibody
- E3 ubiquitin-protein ligase RNF8 antibody
see all
画像
-
All lanes : Anti-RNF8 antibody [EPR6311(2)] (ab128872) at 1/1000 dilution
Lane 1 : Human fetal brain lysate
Lane 2 : Human fetal thymus lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 55 kDa -
All lanes : Anti-RNF8 antibody [EPR6311(2)] (ab128872) at 1/1000 dilution
Lane 1 : Mouse breast tissue lysate
Lane 2 : Mouse ovary tissue lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 103 seconds
-
Anti-RNF8 antibody [EPR6311(2)] (ab128872) at 1/1000 dilution + PC-12 (rat adrenal gland pheochromocytoma) whole cell lysate at 20 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 55 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?Blocking and diluting buffer and concentration: 5% NFDM/TBST
Exposure time: 3 minutes
This blot was developed using a higher sensitivity ECL substrate
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (0)
ab128872 は論文での使用が確認できていません。