Application
ChIP
Sample
Mouse Tissue lysate - nuclear (brown fat)
Negative control
The ChIP was performed with 10 µg of chromatin, 2 µg of ab5131, and 20 µl of Millipore Protein A agarose/salmon Sperm DNA. The immunoprecipitated DNA was quantified on the active Ucp1, Pparg and Fasn promoters, 10kb upstream of the promoter regions were quantified as negative control.
Specification
brown fat
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 9 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.5% formalin in PBS(Ca, Mg fr
Duration of cross-linking step: 9 minute(s) and 0 second(s)
Specification of the cross-linking agent: 0.5% formalin in PBS(Ca, Mg fr
Positive control
The ChIP was performed with 10 µg of chromatin, 2 µg of ab5131, and 20 µl of Millipore Protein A agarose/salmon Sperm DNA. The immunoprecipitated DNA was quantified on the active Ucp1 and Fasn promoters, 10kb upstream of the promoter regions were quantified as negative control.
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP dilution buffer
Additional data
Additional Notes
For detailed protocol and the construction of IP dilution buffer, see Daniel Castellano-Castillo et al, Plos One 2018
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投稿 Nov 20 2018