Application
Western blot
Loading amount
1e+006 cells
Gel Running Conditions
Reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (Breast)
Specification
Breast
Treatment
tnf-a
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Bands
Specific: 230 kDa Non-specific: 220 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
First band is before treatment and the second band is following treatment.
Additional data
Additional Notes
The bands in the WB are 230 kDa. One non specific band is seen just beneath the specific band. Additionally the blot contains several specific bands of other proteins (incubated with other antibodies).
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投稿 Jan 07 2015