Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (Colon)
Specification
Colon
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 4%
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde 4%
Other product details
Incubation time
10 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Additional data
Additional Notes
The locus we performed PCR on is a cytokine gene body. To activate RNA pol2 we treated the cells with TNF-alpha.
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投稿 Dec 17 2014