Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Osteosarcoma)
Permeabilization
Yes - 0.3% tritonX-100
Specification
Osteosarcoma
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Methanol
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/100
Secondary antibody
Dilution
1/300
Name
Non-Abcam antibody was used: anti-mouse
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Host species: Donkey
Clonality: Monoclonal
Conjugation: Cy3 ®
Additional data
Additional Notes
You could see the staining signal inside the nucleus, but the background is also high.
Abcam response
ab5401 has not been validated for use in ICC. Protocol adjustments may improve results.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 May 28 2021