Application
Western blot
Sample
Rabbit Cell lysate - whole cell (Rabbit Reticulocyte)
Gel Running Conditions
Reduced Denaturing (4-12% Bolt Bis-Tris Plus gel)
Loading amount
0.5 µg
Specification
Rabbit Reticulocyte
Blocking step
Li-Cor Odyssey Blocking Buffer (TBS) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Li-Cor Odyssey Blocking Buffer (TBS)
Dilution
1/10000
Secondary antibody
Dilution
1/20000
Name
Non-Abcam antibody was used: IRDye 800CW Goat anti-Rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye® 800CW
Detection
Detection method
Near-Infrared Fluorescence Imaging (NIR)
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 220, 250, 270 kDa Non-specific: 60, 70, 100 kDa
Additional data
Additional Notes
Both anti-phospho S2 antibody (ab5095) and anti-beta actin (ab6276) antibodies were used for the western blot. The anti-phospho S2 antibody (ab5095) itself detected specific bands at 220, 250, 270 kDa (green bands). The 42 kDa red band is the beta actin band (red band).
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投稿 Nov 21 2016