Application
Immunoprecipitation
Sample
Human Cell lysate - whole cell (A549 (human lung epithelium cells))
Total protein in input
1e+007 cells
Immuno-precipitation step
Other - Protein G Dynabeads
Specification
A549 (human lung epithelium cells)
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Pierce™ IP Lysis Buffer
Dilution
1/250
Western Blot antibody
Dilution
1/2000
Name
Additional data
Additional Notes
Lane 1: IP by control mouse IgG
Lane 2: IP by RNAP II Anti-RNA (phosphor S2 CTD) antibody (Abcam ab5095)
Bands (200-210 & 240 KDa) corresponding to RNAP II phosphor S2 CTD forms can be detected in RNAP II phosphor S2 CTD pulldown sample but not control IgG sample. Non-specific bands (100, 80, 60 kDa) also shown in the pulldown sample. Heavy chain and light chain of IP antibodies can be seen around 50 kDa and 25 kDa.
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投稿 Jul 07 2016