Application
ChIP
Sample
Mouse Cell lysate - nuclear (macrophages and fibroblasts)
Specification
macrophages and fibroblasts
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde
Detection step
Real-time PCR
Positive control
cells expressing c-fms (RAW macrophages)
Negative control
cells not expressing c-fms (NIH 3T3 fibroblasts)
Other product details
Concentration
4 µg/ml
Incubation time
0 minute(s)
Additional data
Additional Notes
Nuclear preparation frozen before sonication with probe sonicator (Sonifier). All buffers used contained protease inhibitors.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Aug 15 2006