Anti-RNA Helicase A 抗体 (ab26271)
Key features and details
- Rabbit polyclonal to RNA Helicase A
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RNA Helicase A antibody
RNA Helicase A 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RNA Helicase A -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Monkey, African green monkey -
免疫原
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab26271の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (5) |
Use a concentration of 1 µg/ml. Detects a band of approximately 141,149 kDa (predicted molecular weight: 141 kDa).
This antibody detects a band at 141 kDa in human cell lysates and 149 kDa in mouse cell lysates. |
特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 141,149 kDa (predicted molecular weight: 141 kDa). This antibody detects a band at 141 kDa in human cell lysates and 149 kDa in mouse cell lysates. |
ターゲット情報
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機能
Unwinds double-stranded DNA and RNA in a 3' to 5' direction. Alteration of secondary structure may subsequently influence interactions with proteins or other nucleic acids. Functions as a transcriptional activator. Component of the CRD-mediated complex that promotes MYC mRNA stability. -
配列類似性
Belongs to the DEAD box helicase family. DEAH subfamily.
Contains 2 DRBM (double-stranded RNA-binding) domains.
Contains 1 helicase ATP-binding domain.
Contains 1 helicase C-terminal domain. -
ドメイン
The MTAD domain mediates interaction with the RNA polymerase II holoenzyme. The NTD domain is necessary and sufficient for nucleo-cytoplasmic shuttling and interaction with HRMT1L2 and SMN1. -
翻訳後修飾
Methylated. HRMT1L2 mediated methylation of undefined Arg residues in the NTD is required for nuclear localization.
May be phosphorylated by PRKDC/XRCC7. Phosphorylated upon DNA damage, probably by ATM or ATR. -
細胞内局在
Nucleus > nucleolus. Cytoplasm. Localized in cytoplasmic mRNP granules containing untranslated mRNAs. Can shuttle between nucleus and cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 281115 Cow
- Entrez Gene: 1660 Human
- Entrez Gene: 13211 Mouse
- Omim: 603115 Human
- SwissProt: Q28141 Cow
- SwissProt: Q08211 Human
- SwissProt: O70133 Mouse
- Unigene: 191518 Human
see all -
別名
- ATP dependent RNA helicase A antibody
- ATP-dependent RNA helicase A antibody
- DDX 9 antibody
see all
画像
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All lanes : Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml
Lane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 2 : HEK293 (Human) Whole Cell Lysate
Lane 3 : NIH 3T3 (Mouse) Whole Cell Lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141,149 kDa why is the actual band size different from the predicted?
Additional bands at: 50 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 4 minutesThis blot was produced using a 3-8% Tris Acetate gel under the TA buffer system. The gel was run at 150V for 60 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab26271 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution ab133406.
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Anti-RNA Helicase A antibody (ab26271) at 1 µg/ml + HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG (Alexa Fluor® 680) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 141 kDa
Observed band size: 141 kDa -
All lanes : Anti-RNA Helicase A antibody (ab26271) at 1/1000 dilution
All lanes : Human embryonic stem cell nuclear lysate
Lysates/proteins at 50 µg per lane.
Secondary
All lanes : Donkey anti-Rabbit HRP IgG at 1/10000 dilution
Developed using the ECL technique.
Predicted band size: 141 kDa
Exposure time: 30 seconds
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (48)
ab26271 は 48 報の論文で使用されています。
- Kazuma Y et al. ILF2 enhances the DNA cytosine deaminase activity of tumor mutator APOBEC3B in multiple myeloma cells. Sci Rep 12:2278 (2022). PubMed: 35145187
- Liu Y et al. The CTCF/LncRNA-PACERR complex recruits E1A binding protein p300 to induce pro-tumour macrophages in pancreatic ductal adenocarcinoma via directly regulating PTGS2 expression. Clin Transl Med 12:e654 (2022). PubMed: 35184402
- Beghè C & Gromak N R-Loop Immunoprecipitation: A Method to Detect R-Loop Interacting Factors. Methods Mol Biol 2528:215-237 (2022). PubMed: 35704194
- Wang P et al. Circular RNA circBNC2 inhibits epithelial cell G2-M arrest to prevent fibrotic maladaptive repair. Nat Commun 13:6502 (2022). PubMed: 36316334
- Montavon TC et al. Human DICER helicase domain recruits PKR and modulates its antiviral activity. PLoS Pathog 17:e1009549 (2021). PubMed: 33984068