Name: Anti-RKIP antibody [EPR2875Y]
Brand: RabMAb
SKU: ab76582
Rating: 5 (5 reviews)

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Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR2875Y] to RKIP
Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
Reacts with: Mouse, Rat, Human

製品名

Anti-RKIP antibody [EPR2875Y]
RKIP 一次抗体製品一覧
製品の詳細

Rabbit monoclonal [EPR2875Y] to RKIP
由来種

Rabbit
アプリケーション

適用あり: Flow Cyt, IHC-P, WB, ICC/IFmore details
適用なし: IP
種交差性

交差種: Mouse, Rat, Human
免疫原

Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.
ポジティブ･コントロール
- WB: HeLa, A549, HepG2, A431, mouse brain, mouse testis and rat brain cell lysates; IHC-P: human ovarian carcinoma and brain, mouse kidney, and rat testis tissues; ICC/IF: HepG2 cells; Flow Cyt: HeLa cells.
特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の状態

Liquid
保存方法

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
バッファー

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
Concentration information loading...
精製度

Protein A purified
ポリ/モノ

モノクローナル
クローン名

EPR2875Y
アイソタイプ

IgG
研究分野

Alternative Versions
- Anti-RKIP antibody [EPR2875Y] - BSA and Azide free (ab239887)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HepG2 cytoplasmic extract lysate (ab14659)
Recombinant Protein
- Recombinant Human RKIP protein (ab87892)

Our Abpromise guarantee covers the use of ab76582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション	Abreviews	特記事項
Flow Cyt		1/20.
IHC-P		1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. See IHC antigen retrieval protocols.
WB		1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF		1/50 - 1/100.

追加情報

Is unsuitable for IP.

機能

Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
配列類似性

Belongs to the phosphatidylethanolamine-binding protein family.
細胞内局在

Cytoplasm.
Target information above from: UniProt accession P30086 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
参照データベース
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P70296 Mouse
- SwissProt: P31044 Rat
- Unigene: 433863 Human
- Unigene: 195898 Mouse
- Unigene: 338476 Mouse
- Unigene: 473809 Mouse
- Unigene: 29745 Rat
see all
別名
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
- HCNP antibody
- HCNPpp antibody
- HEL 210 antibody
- HEL S 34 antibody
- HEL S 96 antibody
- Hippocampal cholinergic neurostimulating peptide antibody
- Neuropolypeptide h3 antibody
- PBP antibody
- PEBP antibody
- PEBP 1 antibody
- PEBP-1 antibody
- Pebp1 antibody
- PEBP1_HUMAN antibody
- Phosphatidylethanolamine binding protein antibody
- Phosphatidylethanolamine binding protein 1 antibody
- Prostatic binding protein antibody
- Prostatic-binding protein antibody
- R kip antibody
- Raf kinase inhibitor protein antibody
- Raf kinase inhibitory protein antibody
- RKIP antibody
see all

Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/1000 dilution (Purified)

Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Mouse testis lysates
Lane 5 : Rat brain lysates

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa
Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RKIP with purified ab76582 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)

Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with purified ab76582 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor^® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified)

Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate

Lysates/proteins at 10 µg per lane.

Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution

Predicted band size: 21 kDa
Observed band size: 21 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582 (unpurified).

Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)

Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified) + Recombinant Human RKIP protein (ab87892) at 0.1 µg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Developed using the ECL technique.

Performed under reducing conditions.

Predicted band size: 21 kDa

Exposure time: 30 seconds
Anti-RKIP antibody [EPR2875Y] (ab76582)

Flow cytometry protocols
Immunoprecipitation protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols
Western blot protocols

Click here to view the general protocols

Datasheet

ab76582 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab76582 は 12 報の論文で使用されています。

You H et al. Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer. Sci Adv 6:eaax6040 (2020). PubMed: 32076639
Raquel-Cunha A et al. Current Status of Raf Kinase Inhibitor Protein (RKIP) in Lung Cancer: Behind RTK Signaling. Cells 8:N/A (2019). PubMed: 31083461
Xie SY et al. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer. Onco Targets Ther 10:5605-5619 (2017). PubMed: 29200875
Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). PubMed: 29163033
Papale M et al. Urinary RKIP/p-RKIP is a potential diagnostic and prognostic marker of clear cell renal cell carcinoma. Oncotarget 8:40412-40424 (2017). PubMed: 28418894

View all Publications for this product

レビューと Q&A

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レビューを送る質問を送る

1-5 of 5 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (A2058 melanoma cell line)

Gel Running Conditions

Reduced Denaturing (12% Acrisbys Gel)

Loading amount

20 µg

Specification

A2058 melanoma cell line

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Cristina Penas Lago

Verified customer

投稿 Mar 06 2018

Application

Immunocytochemistry/ Immunofluorescence

Sample

Mouse Cell (neuronal)

Specification

neuronal

Fixative

Formaldehyde

Permeabilization

Yes - a. PBS + 0.2% Triton x-100

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

投稿 Aug 31 2012

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (NEURONAL)

Specification

NEURONAL

Fixative

Formaldehyde

Permeabilization

Yes - a. PBS + 0.2% Triton x-100

Blocking step

BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C

Abcam user community

Verified customer

投稿 Aug 30 2012

Application

Western blot

Sample

Mouse Cell lysate - whole cell (neuronal)

Loading amount

50 µg

Specification

neuronal

Gel Running Conditions

Non-reduced Denaturing

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

投稿 Aug 30 2012

Application

Western blot

Sample

Human Cell lysate - whole cell (neuronal)

Loading amount

50 µg

Specification

neuronal

Gel Running Conditions

Non-reduced Denaturing

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C

Abcam user community

Verified customer

投稿 Aug 30 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

製品の概要

製品名

製品の詳細

由来種

アプリケーション

種交差性

免疫原

ポジティブ･コントロール

特記事項

製品の特性

製品の状態

保存方法

バッファー

精製度

ポリ/モノ

クローン名

アイソタイプ

研究分野

関連製品

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

アプリケーション

ターゲット情報

機能

配列類似性

細胞内局在

参照データベース

別名

画像

プロトコール

データシートおよび資料

参考文献 (12)

レビューと Q&A

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