Anti-RKIP 抗体 [EPR2875Y] (ab76582)
製品の概要
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製品名Anti-RKIP antibody [EPR2875Y]
RKIP 一次抗体 製品一覧 -
製品の詳細Rabbit monoclonal [EPR2875Y] to RKIP
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由来種Rabbit
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アプリケーション適用あり: Flow Cyt, IHC-P, IHC-Fr, WB, IP, ICC/IFmore details
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種交差性交差種: Mouse, Rat, Human
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免疫原
Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.
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ポジティブ･コントロール
- WB: HeLa, A549, HepG2 and A431 cell lysate. IHC-P: Human brain tissue. Flow Cyt: HeLa cells.
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
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バッファーpH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant -
精製度Tissue culture supernatant
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ポリ/モノモノクローナル
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クローン名EPR2875Y
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アイソタイプIgG
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研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab76582 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| Flow Cyt | 1/200. | |
| IHC-P | 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| IHC-Fr | Use at an assay dependent concentration. PubMed: 22542739 | |
| WB | 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa). | |
| IP | 1/10. | |
| ICC/IF | 1/100. |
ターゲット情報
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機能Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor. -
配列類似性Belongs to the phosphatidylethanolamine-binding protein family.
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細胞内局在Cytoplasm.
- Information by UniProt
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参照データベース
- Entrez Gene: 5037 Human
- Entrez Gene: 23980 Mouse
- Entrez Gene: 29542 Rat
- Omim: 604591 Human
- SwissProt: P30086 Human
- SwissProt: P70296 Mouse
- SwissProt: P31044 Rat
- Unigene: 433863 Human
see all -
別名
- Epididymis luminal protein 210 antibody
- Epididymis secretory protein Li 34 antibody
- Epididymis secretory protein Li 96 antibody
see all
画像
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.co.jp/ps/products/76/ab76582/Images/ab76582ihc.gif)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582. -
![Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.co.jp/ps/products/76/ab76582/Images/ab76582wb.gif)
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution
Lane 1 : HeLa cell lysate
Lane 2 : A549 cell lysate
Lane 3 : HepG2 cell lysate
Lane 4 : A431 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : goat anti-rabbit HRP at 1/2000 dilution
Predicted band size: 21 kDa
Observed band size: 21 kDa -
![Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.co.jp/ps/products/76/ab76582/Images/ab76582-5-ab76582FC1.jpg)
Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with ab76582 at a 1/200 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.
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![Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)](https://www.abcam.co.jp/ps/products/76/ab76582/Images/PBP-Primary-antibodies-ab76582-2.jpg)
Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution +Recombinant Human RKIP protein (ab87892) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 30 seconds
データシートおよび資料
プロトコール
参考文献
This product has been referenced in:
- Xie SY et al. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer. Onco Targets Ther 10:5605-5619 (2017). Read more (PubMed: 29200875) »
- Wang Z et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). Read more (PubMed: 29163033) »
