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  1. Link

    rkip-antibody-epr2875y-ab76582.pdf

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リコンビナントRabMAb

Anti-RKIP 抗体 [EPR2875Y] (ab76582)

  • Datasheet
Reviews (5) Submit a question References (12)

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Abpromise

保証された製品品質、優れたカスタマー・サポート。 詳細はこちら。

Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
  • Anti-RKIP antibody [EPR2875Y] (ab76582)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR2875Y] to RKIP
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

こちらの製品もご検討ください

二次抗体
Product image
Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
タンパク質
Product image
Recombinant Human RKIP protein (ab87892)

関連製品

製品の概要

  • 製品名

    Anti-RKIP antibody [EPR2875Y]
    RKIP 一次抗体 製品一覧
  • 製品の詳細

    Rabbit monoclonal [EPR2875Y] to RKIP
  • 由来種

    Rabbit
  • アプリケーション

    適用あり: Flow Cyt, IHC-P, WB, ICC/IFmore details
    適用なし: IP
  • 種交差性

    交差種: Mouse, Rat, Human
  • 免疫原

    Synthetic peptide within Human RKIP aa 50-150. The exact sequence is proprietary.

  • ポジティブ・コントロール

    • WB: HeLa, A549, HepG2, A431, mouse brain, mouse testis and rat brain cell lysates; IHC-P: human ovarian carcinoma and brain, mouse kidney, and rat testis tissues; ICC/IF: HepG2 cells; Flow Cyt: HeLa cells.
  • 特記事項

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • バッファー

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • 精製度

    Protein A purified
  • ポリ/モノ

    モノクローナル
  • クローン名

    EPR2875Y
  • アイソタイプ

    IgG
  • 研究分野

    • Cell Biology
    • Cell Cycle
    • Kinases/Phosphatases
    • Other
    • Cell Biology
    • Cell Cycle
    • Cell Division
    • Spindle
    • Neuroscience
    • Neurotransmitter
    • Acetylcholine
    • Neuroscience
    • Neurotransmitter
    • Neuropeptides
    • More Neuropeptides
    • Epigenetics and Nuclear Signaling
    • Cell cycle
    • Kinases/Phosphatases
    • Other
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other

関連製品

  • Alternative Versions

    • Anti-RKIP antibody [EPR2875Y] - BSA and Azide free (ab239887)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HepG2 cytoplasmic extract lysate (ab14659)
  • Recombinant Protein

    • Recombinant Human RKIP protein (ab87892)

アプリケーション

Our Abpromise guarantee covers the use of ab76582 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
Flow Cyt 1/20.
IHC-P 1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

WB 1/1000 - 1/5000. Detects a band of approximately 21 kDa (predicted molecular weight: 21 kDa).
ICC/IF 1/50 - 1/100.
  • 追加情報
    Is unsuitable for IP.
  • ターゲット情報

    • 機能

      Binds ATP, opioids and phosphatidylethanolamine. Has lower affinity for phosphatidylinositol and phosphatidylcholine. Serine protease inhibitor which inhibits thrombin, neuropsin and chymotrypsin but not trypsin, tissue type plasminogen activator and elastase.
      HCNP may be involved in the function of the presynaptic cholinergic neurons of the central nervous system. HCNP increases the production of choline acetyltransferase but not acetylcholinesterase. Seems to be mediated by a specific receptor.
    • 配列類似性

      Belongs to the phosphatidylethanolamine-binding protein family.
    • 細胞内局在

      Cytoplasm.
    • Target information above from: UniProt accession P30086 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • 参照データベース

      • Entrez Gene: 5037 Human
      • Entrez Gene: 23980 Mouse
      • Entrez Gene: 29542 Rat
      • Omim: 604591 Human
      • SwissProt: P30086 Human
      • SwissProt: P70296 Mouse
      • SwissProt: P31044 Rat
      • Unigene: 433863 Human
      • Unigene: 195898 Mouse
      • Unigene: 338476 Mouse
      • Unigene: 473809 Mouse
      • Unigene: 29745 Rat
      see all
    • 別名

      • Epididymis luminal protein 210 antibody
      • Epididymis secretory protein Li 34 antibody
      • Epididymis secretory protein Li 96 antibody
      • HCNP antibody
      • HCNPpp antibody
      • HEL 210 antibody
      • HEL S 34 antibody
      • HEL S 96 antibody
      • Hippocampal cholinergic neurostimulating peptide antibody
      • Neuropolypeptide h3 antibody
      • PBP antibody
      • PEBP antibody
      • PEBP 1 antibody
      • PEBP-1 antibody
      • Pebp1 antibody
      • PEBP1_HUMAN antibody
      • Phosphatidylethanolamine binding protein antibody
      • Phosphatidylethanolamine binding protein 1 antibody
      • Prostatic binding protein antibody
      • Prostatic-binding protein antibody
      • R kip antibody
      • Raf kinase inhibitor protein antibody
      • Raf kinase inhibitory protein antibody
      • RKIP antibody
      see all

    画像

    • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
      All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/1000 dilution (Purified)

      Lane 1 : HeLa (Human cervix adenocarcinoma epithelial cell) whole cell lysates
      Lane 2 : A549 (Human lung carcinoma epithelial cell) whole cell lysates
      Lane 3 : Mouse brain lysates
      Lane 4 : Mouse testis lysates
      Lane 5 : Rat brain lysates

      Lysates/proteins at 20 µg per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa

    • Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Immunocytochemistry/ Immunofluorescence - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Immunocytochemistry/ Immunofluorescence analysis of HepG2 (Human hepatocellular carcinoma epithelial cell) cells labeling RKIP with purified ab76582 at 1:50 dilution (2.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat testis tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Flow Cytometry - Anti-RKIP antibody [EPR2875Y] (ab76582)

      Flow cytometry analysis of HeLa (human cervix adenocarcinoma) cells labeling RKIP (red) with purified ab76582 at a 1/20 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (ab172730). Blue (unlabeled control) - Cells without incubation with the primary and secondary antibodies.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse kidney tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human ovarian carcinoma tissue sections labeling RKIP with purified ab76582 at 1/500 dilution (0.26 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.

    • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
      All lanes : Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified)

      Lane 1 : HeLa cell lysate
      Lane 2 : A549 cell lysate
      Lane 3 : HepG2 cell lysate
      Lane 4 : A431 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 21 kDa
      Observed band size: 21 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-RKIP antibody [EPR2875Y] (ab76582)

      Staining of formalin-fixed, paraffin-embedded human brain tissue using 1/250 ab76582 (unpurified).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Western blot - Anti-RKIP antibody [EPR2875Y] (ab76582)
      Anti-RKIP antibody [EPR2875Y] (ab76582) at 1/5000 dilution (unpurified) + Recombinant Human RKIP protein (ab87892) at 0.1 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 21 kDa


      Exposure time: 30 seconds
    • Anti-RKIP antibody [EPR2875Y] (ab76582)
      Anti-RKIP antibody [EPR2875Y] (ab76582)

    プロトコール

    • Flow cytometry protocols
    • Immunoprecipitation protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols
    • Western blot protocols

    Click here to view the general protocols

    データシートおよび資料

    • Datasheet
  • 参考文献 (12)

    ab76582 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab76582 は 12 報の論文で使用されています。

    • You H  et al. Sight and switch off: Nerve density visualization for interventions targeting nerves in prostate cancer. Sci Adv 6:eaax6040 (2020). PubMed: 32076639
    • Raquel-Cunha A  et al. Current Status of Raf Kinase Inhibitor Protein (RKIP) in Lung Cancer: Behind RTK Signaling. Cells 8:N/A (2019). PubMed: 31083461
    • Xie SY  et al. RKIP reduction enhances radioresistance by activating the Shh signaling pathway in non-small-cell lung cancer. Onco Targets Ther 10:5605-5619 (2017). PubMed: 29200875
    • Wang Z  et al. Phosphorylation at S153 as a Functional Switch of Phosphatidylethanolamine Binding Protein 1 in Cerebral Ischemia-Reperfusion Injury in Rats. Front Mol Neurosci 10:358 (2017). PubMed: 29163033
    • Papale M  et al. Urinary RKIP/p-RKIP is a potential diagnostic and prognostic marker of clear cell renal cell carcinoma. Oncotarget 8:40412-40424 (2017). PubMed: 28418894
    View all Publications for this product

    レビューと Q&A

    Show All レビュー Q&A
    レビューを送る 質問を送る

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-PBP antibody [EPR2875Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (A2058 melanoma cell line)
    Gel Running Conditions
    Reduced Denaturing (12% Acrisbys Gel)
    Loading amount
    20 µg
    Specification
    A2058 melanoma cell line
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
    Read More

    Cristina Penas Lago

    Verified customer

    投稿 Mar 06 2018

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PBP antibody [EPR2875Y]

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (neuronal)
    Specification
    neuronal
    Fixative
    Formaldehyde
    Permeabilization
    Yes - a. PBS + 0.2% Triton x-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 31 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-PBP antibody [EPR2875Y]

    Good
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (NEURONAL)
    Specification
    NEURONAL
    Fixative
    Formaldehyde
    Permeabilization
    Yes - a. PBS + 0.2% Triton x-100
    Blocking step
    BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 30 2012

    Western blot abreview for Anti-PBP antibody [EPR2875Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (neuronal)
    Loading amount
    50 µg
    Specification
    neuronal
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 30 2012

    Western blot abreview for Anti-PBP antibody [EPR2875Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (neuronal)
    Loading amount
    50 µg
    Specification
    neuronal
    Gel Running Conditions
    Non-reduced Denaturing
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: rt°C
    Read More

    Abcam user community

    Verified customer

    投稿 Aug 30 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

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