Anti-RIP3 (phospho S232) 抗体 [EPR9516(N)-25] (ab195117)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9516(N)-25] to RIP3 (phospho S232)
- Suitable for: ELISA, WB, Dot blot
- Reacts with: Mouse
Related conjugates and formulations
製品の概要
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製品名
Anti-RIP3 (phospho S232) antibody [EPR9516(N)-25]
RIP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9516(N)-25] to RIP3 (phospho S232) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, WB, Dot blotmore details
適用なし: IHC-P or IP -
種交差性
交差種: Mouse -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: L-929 whole cell lysate treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 8 h and then harvested; Untreated L-929 whole cell lysate; TSE (WT) whole cell lysate treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 12 h and then harvested; TSE (KO MLKL) whole cell lysate treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 12 h and then harvested.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9516(N)-25 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab195117の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ELISA | (1) |
Use at an assay dependent concentration.
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WB | (8) |
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa).
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Dot blot |
Use at an assay dependent concentration.
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000. Detects a band of approximately 53 kDa (predicted molecular weight: 53 kDa). |
Dot blot
Use at an assay dependent concentration. |
ターゲット情報
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機能
Promotes apoptosis. -
組織特異性
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 56532 Mouse
- SwissProt: Q9QZL0 Mouse
- Unigene: 46612 Mouse
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別名
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
画像
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All lanes : Anti-RIP3 (phospho S232) antibody [EPR9516(N)-25] (ab195117) at 1/10000 dilution
Lane 1 : Untreated L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate
Lane 2 : L-929 (Mouse connective tissue fibroblast cell line) whole cell lysate treated with phosphatase
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
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All lanes : Anti-RIP3 (phospho S232) antibody [EPR9516(N)-25] (ab195117) at 1/2000 dilution
Lane 1 : Untreated TSE (WT) whole cell lysate
Lane 2 : TSE (WT) whole cell lysate treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 12 h and then harvested
Lane 3 : Untreated TSE (KO RIP3) whole cell lysate
Lane 4 : TSE (KO RIP3) whole cell lysate treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 12 h and then harvested
Lane 5 : Untreated TSE (KO MLKL) whole cell lysate
Lane 6 : TSE (KO MLKL) whole cell lysate treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 12 h and then harvested
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/1000 dilution
Predicted band size: 53 kDa
Observed band size: 53 kDa
Exposure time: 30 secondsBlocking/Dilution buffer: 5% NFDM/TBST.
Lysates were provided by Xiaodong Wang, National Institute of Biological Sciences, Beijing
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Primary antibody: ab195117 at 1:1000 dilution (1.6μg/ml), secondary antibody: Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1:1000 dilution, Blocking and dilution buffer: 5% NFDM/TBST, Lane 1: RIP3 (phospho S232) phospho peptide, Lane 2: RIP3 Non-phospho peptide, Exposure time: 3 minutes.
We observed weak binding to the non-phospho peptide at very high concentrations.
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Serially diluted ab195117 was bound to immobilized phospho or non-phospho (control) peptide demonstrating minimal cross-reactivity to the non-phosphorylated residue. Antigen - RIP3 (phospho S232) phospho peptide;RIP3 non-phospho peptide at 250 ng/ml. Primary antibody concentration range: 0 - 500 ng/ml. Secondary antibody - Alkaline Phosphatase-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at 1:2500.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (58)
ab195117 は 58 報の論文で使用されています。
- Li X et al. Caspase-8 auto-cleavage regulates programmed cell death and collaborates with RIPK3/MLKL to prevent lymphopenia. Cell Death Differ 29:1500-1512 (2022). PubMed: 35064213
- Sun S et al. Adenosine Kinase Inhibition Prevents Severe Acute Pancreatitis via Suppressing Inflammation and Acinar Cell Necroptosis. Front Cell Dev Biol 10:827714 (2022). PubMed: 35281076
- Cai Z et al. Linear ubiquitination modification of NR6A1 by LUBAC inhibits RIPK3 kinase activity and attenuates apoptosis of vascular smooth muscle cells. J Biochem Mol Toxicol 36:e23091 (2022). PubMed: 35543488
- Liu C et al. Necrostatin-1 decreases necroptosis and inflammatory markers after intraventricular hemorrhage in mice. Neural Regen Res 17:2710-2716 (2022). PubMed: 35662218
- Chung H et al. Curcumin Blocks High Glucose-Induced Podocyte Injury via RIPK3-Dependent Pathway. Front Cell Dev Biol 10:800574 (2022). PubMed: 35706905