Anti-RIP3 (phospho S227) 抗体 [EPR9627] (ab209384)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR9627] to RIP3 (phospho S227)
- Suitable for: ELISA, Dot blot, WB
- Reacts with: Human
Related conjugates and formulations
製品の概要
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製品名
Anti-RIP3 (phospho S227) antibody [EPR9627]
RIP3 一次抗体 製品一覧 -
製品の詳細
Rabbit monoclonal [EPR9627] to RIP3 (phospho S227) -
由来種
Rabbit -
アプリケーション
適用あり: ELISA, Dot blot, WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- WB: HT-29 treated with 20 ng/ml TNF alpha,100 nM Smac mimetic, and 20 µM z-VAD for 8 h and then harvested whole cell lysate.
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特記事項
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EPR9627 -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab209384の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
ELISA |
Use at an assay dependent concentration.
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Dot blot |
1/1000.
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WB |
1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa).
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特記事項 |
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ELISA
Use at an assay dependent concentration. |
Dot blot
1/1000. |
WB
1/2000. Detects a band of approximately 70 kDa (predicted molecular weight: 57 kDa). |
ターゲット情報
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機能
Promotes apoptosis. -
組織特異性
Detected at lower levels in heart, placenta, lung and kidney. Isoform 3 is significantly increased in colon and lung cancers. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 11035 Human
- Omim: 605817 Human
- SwissProt: Q9Y572 Human
- Unigene: 268551 Human
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別名
- Receptor interacting protein 3 antibody
- Receptor interacting serine threonine kinase 3 antibody
- Receptor interacting serine/threonine protein kinase 3 antibody
see all
画像
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All lanes : Anti-RIP3 (phospho S227) antibody [EPR9627] (ab209384) at 1/1000 dilution
Lane 1 : HT-29 (Human colorectal adenocarcinoma epithelial cell) treated with 20 ng/ml TNF alpha, 100 nM Smac mimetic and 20µM VAD-FMK for 6 h cell lysate
Lane 2 : HT-29 (Human colorectal adenocarcinoma epithelial cell) cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 57 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 100 secondsBlocking/Diluting buffer and concentration 5% NFDM/TBST
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Dot blot analysis of RIP3 (phospho S227) phospho peptide (Lane 1) and RIP3 non-phospho peptide (Lane 2) labeled using ab209384 at 1/1000 dilution, followed by Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated (ab97051) at 1/100,000 dilution.
Blocking and diluting buffer: 5% NFDM/TBST.
Exposure time: 10 seconds.
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Direct ELISA antibody dose-response curve using ab209384. Antigen concentration of 1000 ng/mL and primary antibody concentration of 0-1000 ng/mL. A Alkaline Phosphatase-conjugated AffiniPure -conjugated goat anti-rabbit IgG (1/2500) was used as the secondary antibody.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (65)
ab209384 は 65 報の論文で使用されています。
- Kung YA et al. Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis. mBio 13:e0271721 (2022). PubMed: 35038927
- Huang WY et al. TNFα-mediated necroptosis in brain endothelial cells as a potential mechanism of increased seizure susceptibility in mice following systemic inflammation. J Neuroinflammation 19:29 (2022). PubMed: 35109859
- Jacobsen AV et al. The Lck inhibitor, AMG-47a, blocks necroptosis and implicates RIPK1 in signalling downstream of MLKL. Cell Death Dis 13:291 (2022). PubMed: 35365636
- Lee AR et al. Mitochondrial Transplantation Ameliorates the Development and Progression of Osteoarthritis. Immune Netw 22:e14 (2022). PubMed: 35573148
- Wang F et al. Homoharringtonine combined with cladribine and aclarubicin (HCA) in acute myeloid leukemia: A new regimen of conventional drugs and its mechanism. Oxid Med Cell Longev 2022:8212286 (2022). PubMed: 35873796