Anti-RIP2 抗体 (ab8428)
Key features and details
- Rabbit polyclonal to RIP2
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RIP2 antibody
RIP2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RIP2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide:
PTIPYHKLADLRYLSRGASG
, corresponding to amino acids 11/30 of Human RIP2. (Peptide available as ab8463.) -
ポジティブ･コントロール
- A431 or K562 whole cell lysate An approximately60 kDa band can be detected.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.2
Preservative: 0.02% Sodium azide -
Concentration information loading... -
精製度
Affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab8428の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
1/1000. Detects a band of approximately 60 kDa.
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| ICC/IF |
Use a concentration of 10 µg/ml.
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| 特記事項 |
|---|
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WB
1/1000. Detects a band of approximately 60 kDa. |
|
ICC/IF
Use a concentration of 10 µg/ml. |
ターゲット情報
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機能
Activates pro-caspase-1 and pro-caspase-8. Potentiates CASP8-mediated apoptosis. Activates NF-kappa-B. -
組織特異性
Detected in heart, brain, placenta, lung, peripheral blood leukocytes, spleen, kidney, testis, prostate, pancreas and lymph node. -
配列類似性
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family.
Contains 1 CARD domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR.
Ubiquitinated; undergoes 'Lys-63'-linked polyubiquitination catalyzed by ITCH. -
細胞内局在
Cytoplasm. - Information by UniProt
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参照データベース
- Entrez Gene: 8767 Human
- Entrez Gene: 192656 Mouse
- Omim: 603455 Human
- SwissProt: O43353 Human
- SwissProt: P58801 Mouse
- Unigene: 103755 Human
- Unigene: 112765 Mouse
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別名
- CARD 3 antibody
- CARD carrying kinase antibody
- CARD containing ICE associated kinase antibody
see all
画像
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Immunocytochemistry/ Immunofluorescence - Anti-RIP2 antibody (ab8428)Immunocytochemistry/ Immunofluorescence of K562 cells staining RIP2 with ab8428 at 20µg/ml
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Western blot - Anti-RIP2 antibody (ab8428)Western Blot of HeLa (1), Ramos (2) and EL4 (3) cell lysates labeling RIP2 with Anti-RIP2 antibody (ab8428) at 1µg/ml. -
Western blot - Anti-RIP2 antibody (ab8428)All lanes : Anti-RIP2 antibody (ab8428) at 1/1000 dilution
Lane 1 : A431 whole cell lysate
Lane 2 : K562 whole cell lysate
Observed band size: 60 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-RIP2 antibody (ab8428)Ab8428 at 20μg/ml staining RIP2 in K562 cells by ICC/IF (Immunocytochemistry/Immunofluorescence).
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Immunocytochemistry/ Immunofluorescence - Anti-RIP2 antibody (ab8428)ab8428 at 10µg/ml staining RIP2 in A431 cells by ICC/IF
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab8428 は 6 報の論文で使用されています。
- Wang SS et al. NLRC4 gene silencing-dependent blockade of NOD-like receptor pathway inhibits inflammation, reduces proliferation and increases apoptosis of dendritic cells in mice with septic shock. Aging (Albany NY) 13:1440-1457 (2021). PubMed: 33406504
- He Y et al. Sustained Exposure to Helicobacter pylori Lysate Inhibits Apoptosis and Autophagy of Gastric Epithelial Cells. Front Oncol 10:581364 (2020). PubMed: 33194715
- Shimada K et al. T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation. Immunity 49:873-885.e7 (2018). PubMed: 30366765
- Wang H et al. Rip2 Is Required for Nod2-Mediated Lysozyme Sorting in Paneth Cells. J Immunol 198:3729-3736 (2017). PubMed: 28330897
- Emmerich CH et al. Lys63/Met1-hybrid ubiquitin chains are commonly formed during the activation of innate immune signalling. Biochem Biophys Res Commun 474:452-461 (2016). PubMed: 27133719
- Hollenbach E et al. Inhibition of RICK/nuclear factor-kappaB and p38 signaling attenuates the inflammatory response in a murine model of Crohn disease. J Biol Chem 280:14981-8 (2005). PubMed: 15691843