Anti-RING2 / RING1B / RNF2 抗体 (ab101273)
Key features and details
- Rabbit polyclonal to RING2 / RING1B / RNF2
- Suitable for: WB, IP, ICC/IF, IHC-P, ChIP-sequencing
- Reacts with: Mouse, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RING2 / RING1B / RNF2 antibody
RING2 / RING1B / RNF2 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RING2 / RING1B / RNF2 -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, ICC/IF, IHC-P, ChIP-sequencingmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Rat, Rabbit, Horse, Chicken, Cow, Dog, Turkey, Pig, Xenopus laevis, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Xenopus tropicalis, Platypus -
免疫原
Synthetic peptide corresponding to Human RING2/ RING1B/ RNF2 aa 150-250.
Database link: Q99496 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab101273の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 37 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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ICC/IF | (1) |
Use at an assay dependent concentration.
Use with methanol or paraformaldehyde fixed cells. |
IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ChIP-sequencing |
Use at an assay dependent concentration.
10 µg |
特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 37 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
ICC/IF
Use at an assay dependent concentration. Use with methanol or paraformaldehyde fixed cells. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ChIP-sequencing
Use at an assay dependent concentration. 10 µg |
ターゲット情報
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機能
E3 ubiquitin-protein ligase that mediates monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. May be involved in the initiation of both imprinted and random X inactivation. Essential component of the Polycomb group (PcG) multiprotein PRC1 complex, a complex required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex act via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. E3 ubiquitin-protein ligase activity is enhanced by BMI1/PCGF4. Acts as the main E3 ubiquitin ligase on histone H2A of the PRC1 complex, while RING1 may rather act as a modulator of RNF2/RING2 activity. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 1 RING-type zinc finger. -
翻訳後修飾
Polyubiquitinated in the presence of UBE2D3 (in vitro).
Monoubiquitinated, by auto-ubiquitination. -
細胞内局在
Nucleus. Chromosome. Enriched on inactive X chromosome (Xi) in female trophoblast stem (TS) cells as well as differentiating embryonic stem (ES) cells. The enrichment on Xi is transient during TS and ES cell differentiation. The association with Xi is mitotically stable in non-differentiated TS cells. - Information by UniProt
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参照データベース
- Entrez Gene: 540090 Cow
- Entrez Gene: 480042 Dog
- Entrez Gene: 6045 Human
- Entrez Gene: 19821 Mouse
- Entrez Gene: 100174504 Orangutan
- Entrez Gene: 304850 Rat
- Omim: 608985 Human
- SwissProt: Q99496 Human
see all -
別名
- BAP 1 antibody
- BAP1 antibody
- DING antibody
see all
画像
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All lanes : Anti-RING2 / RING1B / RNF2 antibody (ab101273) at 0.1 µg/ml
Lane 1 : K562 whole cell lysate at 50 µg
Lane 2 : K562 whole cell lysate at 15 µg
Lane 3 : K562 whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 37 kDa
Exposure time: 30 seconds -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate carcinoma tissue labelling RING2 / RING1B / RNF2 with ab101273 at 1/1000 (1µg/ml).
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Detection of RING2 / RING1B / RNF2 in Immunoprecipitates of K562 whole cell lysate (1 mg for IP, 20% of IP loaded) using ab101273 at 3 µg/mg lysate for IP and at 1 µg/ml for subsequent Western blot detection. Detection: Chemiluminescence with an exposure time of 30 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (18)
ab101273 は 18 報の論文で使用されています。
- Desai D & Pethe P ChIP-qPCR for Polycomb Group Proteins During Neuronal Differentiation of Human Pluripotent Stem Cells. Methods Mol Biol 2520:117-133 (2022). PubMed: 33945141
- Sawai A et al. PRC1 sustains the integrity of neural fate in the absence of PRC2 function. Elife 11:N/A (2022). PubMed: 34994686
- Mizukoshi M et al. In vivo cell proliferation analysis and cell-tracing reveal the global cellular dynamics of periodontal ligament cells under mechanical-loading. Sci Rep 11:9813 (2021). PubMed: 33963224
- Bakhoum MF et al. Loss of polycomb repressive complex 1 activity and chromosomal instability drive uveal melanoma progression. Nat Commun 12:5402 (2021). PubMed: 34518527
- Stielow B et al. The SAM domain-containing protein 1 (SAMD1) acts as a repressive chromatin regulator at unmethylated CpG islands. Sci Adv 7:N/A (2021). PubMed: 33980486