Anti-RIL 抗体 (ab6045)
Key features and details
- Goat polyclonal to RIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-RIL antibody
RIL 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RIL -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chicken, Cow -
免疫原
Synthetic peptide:
DVVAVYPNAKVELV
, corresponding to C terminal amino acids 317-330 of Human RIL. -
特記事項
GenBank Accession Number – NP_003678.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
l -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab6045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
---|---|---|
WB |
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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組織特異性
Isoform 2 is found in brain. -
配列類似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
翻訳後修飾
Phosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13. - Information by UniProt
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参照データベース
- Entrez Gene: 395643 Chicken
- Entrez Gene: 515410 Cow
- Entrez Gene: 8572 Human
- Entrez Gene: 30794 Mouse
- Omim: 603422 Human
- SwissProt: Q9PW72 Chicken
- SwissProt: Q3T005 Cow
- SwissProt: P50479 Human
see all -
別名
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all
画像
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Anti-RIL antibody (ab6045) at 0.2 µg/ml + Lysate prepared from A431 cells. (RIPA buffer, 35µg total protein per lane).
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 1 hour
ab6045 at 0.2µ g/ml staining approximately 37kDa band of RIL in A431 Lysate by Western blot (ECL).
プロトコール
データシートおよび資料
参考文献 (3)
ab6045 は 3 報の論文で使用されています。
- Miyazaki K et al. CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res 318:1716-25 (2012). WB, ICC/IF ; Rat . PubMed: 22659164
- Guryanova OA et al. Actin Cytoskeleton Remodeling by the Alternatively Spliced Isoform of PDLIM4/RIL Protein. J Biol Chem 286:26849-59 (2011). WB . PubMed: 21636573
- Iida Y et al. Localization of reversion-induced LIM protein (RIL) in the rat central nervous system. Acta Histochem Cytochem 42:9-14 (2009). WB, IHC-FoFr ; Rat . PubMed: 19293990
レビューと Q&A
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