Anti-RIL 抗体 (ab6045)
Key features and details
- Goat polyclonal to RIL
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RIL antibody
RIL 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to RIL -
由来種
Goat -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Chicken, Cow
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免疫原
Synthetic peptide:
DVVAVYPNAKVELV
, corresponding to C terminal amino acids 317-330 of Human RIL. -
特記事項
GenBank Accession Number – NP_003678.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
l -
Concentration information loading... -
精製度
Immunogen affinity purified -
特記事項（精製）
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
Our Abpromise guarantee covers the use of ab6045 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
追加情報
IHC-FoFr: Use at an assay dependent dilution (PMID 19293990).
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
WB: Use at a concentration of 0.1 - 0.5 µg/ml. Detects a band of approximately 37 kDa (predicted molecular weight: 35 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
ターゲット情報
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組織特異性
Isoform 2 is found in brain. -
配列類似性
Contains 1 LIM zinc-binding domain.
Contains 1 PDZ (DHR) domain. -
翻訳後修飾
Phosphorylated on tyrosine residue(s). Can be dephosphorylated by PTPN13. - Information by UniProt
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参照データベース
- Entrez Gene: 395643 Chicken
- Entrez Gene: 515410 Cow
- Entrez Gene: 8572 Human
- Entrez Gene: 30794 Mouse
- Omim: 603422 Human
- SwissProt: Q9PW72 Chicken
- SwissProt: Q3T005 Cow
- SwissProt: P50479 Human
see all -
別名
- Enigma homolog antibody
- LIM domain protein antibody
- LIM domain protein RIL antibody
see all
画像
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Western blot - Anti-RIL antibody (ab6045)Anti-RIL antibody (ab6045) at 0.2 µg/ml + Lysate prepared from A431 cells. (RIPA buffer, 35µg total protein per lane).
Developed using the ECL technique.
Predicted band size: 35 kDa
Exposure time: 1 hour
ab6045 at 0.2µ g/ml staining approximately 37kDa band of RIL in A431 Lysate by Western blot (ECL).
プロトコール
データシートおよび資料
参考文献 (3)
ab6045 は 3 報の論文で使用されています。
- Miyazaki K et al. CLP36 and RIL recruit a-actinin-1 to stress fibers and differentially regulate stress fiber dynamics in F2408 fibroblasts. Exp Cell Res 318:1716-25 (2012). WB, ICC/IF ; Rat . PubMed: 22659164
- Guryanova OA et al. Actin Cytoskeleton Remodeling by the Alternatively Spliced Isoform of PDLIM4/RIL Protein. J Biol Chem 286:26849-59 (2011). WB . PubMed: 21636573
- Iida Y et al. Localization of reversion-induced LIM protein (RIL) in the rat central nervous system. Acta Histochem Cytochem 42:9-14 (2009). WB, IHC-FoFr ; Rat . PubMed: 19293990
