製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to RIG-I/DDX58
  • 由来種
    Rabbit
  • アプリケーション
    適用あり: ICC/IF, IHC-P, WBmore details
  • 種交差性
    交差種: Mouse, Rat, Human
  • 免疫原

    Full length protein corresponding to Human RIG-I/DDX58.

  • ポジティブ・コントロール
    • C2C12 cell lysate. Human uterus and mouse heart tissue lysate.

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab45428 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
IHC-P Use a concentration of 5 µg/ml.
WB Use a concentration of 0.5 - 2 µg/ml. Detects a band of approximately 85 kDa (predicted molecular weight: 107 kDa).

ターゲット情報

  • 機能
    Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV.
  • 組織特異性
    Present in vascular smooth cells (at protein level).
  • 配列類似性
    Belongs to the helicase family.
    Contains 2 CARD domains.
    Contains 1 helicase ATP-binding domain.
    Contains 1 helicase C-terminal domain.
  • ドメイン
    The repressor domain controls homomultimerization and interaction with MAVS.
    The helicase domain is responsible for dsRNA recognition.
    The 2 CARD domains are responsible for interaction with and signaling through MAVS.
    The second CARD domain is the primary site for 'Lys-63'-linked ubiquitination.
  • 翻訳後修飾
    Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.
    Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains.
  • 細胞内局在
    Cytoplasm. Colocalized with TRIM25 at cytoplasmic perinuclear bodies.
  • Information by UniProt
  • 参照データベース
  • 別名
    • Ddx58 antibody
    • DDX58_HUMAN antibody
    • DEAD (Asp Glu Ala Asp) box polypeptide 58 antibody
    • DEAD (Asp Glu Ala Asp/His) box polypeptide antibody
    • DEAD box protein 58 antibody
    • DEAD/H (Asp Glu Ala Asp/His) box polypeptide RIG1 antibody
    • DKFZp434J1111 antibody
    • DKFZp686N19181 antibody
    • FLJ13599 antibody
    • Probable ATP dependent RNA helicase DDX58 antibody
    • Probable ATP-dependent RNA helicase DDX58 antibody
    • Retinoic acid inducible gene 1 protein antibody
    • Retinoic acid-inducible gene 1 protein antibody
    • Retinoic acid-inducible gene I protein antibody
    • RIG I antibody
    • Rig-1 antibody
    • RIG-I antibody
    • RIG1 antibody
    • rigi antibody
    • RLR 1 antibody
    • RNA helicase antibody
    • RNA helicase RIG I antibody
    • SGMRT2 antibody
    see all

画像

  • Lane 1 : Anti-RIG-I/DDX58 antibody (ab45428) at 0.5 µg/ml
    Lane 2 : Anti-RIG-I/DDX58 antibody (ab45428) at 1 µg/ml
    Lane 3 : Anti-RIG-I/DDX58 antibody (ab45428) at 2 µg/ml

    All lanes : C2C12 cell lysate

    Predicted band size: 107 kDa
    Observed band size: 85 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 65 kDa, 70 kDa. We are unsure as to the identity of these extra bands.

  • ICC/IF image of ab45428 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45428, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  • ab45428 at 5 µg/ml staining RIG-I/DDX58 in human heart tissue section by Immunohistochemistry (Formalin/ PFA fixed paraffin-embedded tissue sections).

参考文献

This product has been referenced in:
  • Lee W  et al. Vibrio vulnificus quorum-sensing molecule cyclo(Phe-Pro) inhibits RIG-I-mediated antiviral innate immunity. Nat Commun 9:1606 (2018). Read more (PubMed: 29686409) »
  • Zhu H  et al. RIG-I antiviral signaling drives interleukin-23 production and psoriasis-like skin disease. EMBO Mol Med 9:589-604 (2017). WB, IHC-P, Flow Cyt ; Mouse . Read more (PubMed: 28377495) »
See all 12 Publications for this product

レビューと Q&A

1-5 of 5 Abreviews or Q&A

Application
Western blot
Sample
Mouse Tissue lysate - whole (mouse liver)
Gel Running Conditions
Reduced Denaturing
Loading amount
20 µg
Specification
mouse liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Sep 09 2015

Application
Western blot
Loading amount
25 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (Hep G2)
Specification
Hep G2
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Abcam user community

Verified customer

投稿 Dec 24 2014

Answer

I am very pleased to hear you would like to accept our offer and test ab45428 and ab69983 in IP. Each code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview forIP and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Answer

Thank you very much for your interest in ab45428 and ab69983. To our knowledge, these antibodies have not been tested in IP. Therefore, I can offer a discount off a future purchase if you buy ab45428 and/or ab69983now, test it in IPand submit feedback to us in the form of an Abreview. It doesn't matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody for each antibody you test. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab45428 and/or ab69983 in IP. I will then send a discount code. This code must be issued before purchasing ab45428 and/or ab69983 so please wait for my reply before ordering. 2. Purchase ab45428 and/or ab69983 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibodyordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab45428 and/or ab69983 turns out to be unsuitable for IP, you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa and Mouse Embryonic fibroblast (MEF))
Loading amount
50 µg
Specification
HeLa and Mouse Embryonic fibroblast (MEF)
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

投稿 Aug 21 2007

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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