Anti-RICTOR 抗体 [7B3] (ab104838)
Key features and details
- Mouse monoclonal [7B3] to RICTOR
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG1
製品の概要
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製品名
Anti-RICTOR antibody [7B3]
RICTOR 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [7B3] to RICTOR -
由来種
Mouse -
アプリケーション
適用あり: WB, IHC-P, Flow Cyt, ICC/IFmore details -
種交差性
交差種: Mouse, Human -
免疫原
Purified recombinant fragment of Human RICTOR expressed in E. Coli.
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ポジティブ・コントロール
- HeLa, PANC-1, MOLT4, HepG2 and HEK293 cell lysates. Thyroid gland tissues and Human breast carcinoma. NIH3T3 cells.
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特記事項
This product was changed from ascites to supernatant. Lot no’s high than GR173148-16 are from Tissue Culture Supernatant
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C long term. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
Purified from tissue culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
7B3 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab104838の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/500 - 1/2000. Predicted molecular weight: 192 kDa.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
Use at an assay dependent concentration.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ICC/IF |
1/200 - 1/1000.
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特記事項 |
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WB
1/500 - 1/2000. Predicted molecular weight: 192 kDa. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
Use at an assay dependent concentration. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
1/200 - 1/1000. |
ターゲット情報
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機能
Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Plays an essential role in embryonic growth and development. -
配列類似性
Belongs to the RICTOR family. -
翻訳後修飾
Phosphorylated by MTOR; when part of mTORC2. Phosphorylated at Thr-1135 by RPS6KB1; phosphorylation of RICTOR inhibits mTORC2 and AKT1 signaling. - Information by UniProt
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参照データベース
- Entrez Gene: 253260 Human
- Entrez Gene: 78757 Mouse
- Omim: 609022 Human
- SwissProt: Q6R327 Human
- SwissProt: Q6QI06 Mouse
- Unigene: 407926 Human
- Unigene: 275811 Mouse
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別名
- AVO3 antibody
- AVO3 homolog antibody
- DKFZp686B11164 antibody
see all
画像
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All lanes : Anti-RICTOR antibody [7B3] (ab104838) at 1/500 dilution
Lane 1 : HeLa cell lysate.
Lane 2 : PANC-1 cell lysate.
Lane 3 : MOLT4 cell lysate.
Lane 4 : HepG2 cell lysate.
Lane 5 : HEK293 cell lysate.
Predicted band size: 192 kDa -
ab104838 at 1/200 dilution staining RICTOR in NIH3T3 cells (green) by immunofluorescence. DRAQ5 fluorescent DNA dye is shown in blue. Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).
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ab104838 at 1/200 dilution staining RICTOR in paraffin-embedded thyroid gland tissues by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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ab104838 at 1/200 dilution staining RICTOR in paraffin-fixed Human breast carcinoma by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
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ab104838 used for Flow cytometric analysis of HeLa cells (green). The negative control is shown in purple.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (6)
ab104838 は 6 報の論文で使用されています。
- Chadha R & Meador-Woodruff JH Downregulated AKT-mTOR signaling pathway proteins in dorsolateral prefrontal cortex in Schizophrenia. Neuropsychopharmacology 45:1059-1067 (2020). PubMed: 31952070
- Zhong X et al. S100A8 and S100A9 promote endothelial cell activation through the RAGE-mediated mammalian target of rapamycin complex 2 pathway. Mol Med Rep 22:5293-5303 (2020). PubMed: 33174028
- Wen FF et al. Expression of Raptor and Rictor and their relationships with angiogenesis in colorectal cancer. Neoplasma 67:501-508 (2020). PubMed: 32039630
- Zhang E et al. Minocycline promotes cardiomyocyte mitochondrial autophagy and cardiomyocyte autophagy to prevent sepsis-induced cardiac dysfunction by Akt/mTOR signaling. Apoptosis 24:369-381 (2019). PubMed: 30756206
- Frith JE et al. Mechanically-sensitive miRNAs bias human mesenchymal stem cell fate via mTOR signalling. Nat Commun 9:257 (2018). PubMed: 29343687
- Hütt-Cabezas M et al. Activation of mTORC1/mTORC2 signaling in pediatric low-grade glioma and pilocytic astrocytoma reveals mTOR as a therapeutic target. Neuro Oncol 15:1604-14 (2013). IHC-P ; Human . PubMed: 24203892