Rhodopsin peptide (ab4967)

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Key features and details

  • Purity: > 70% HPLC
  • Suitable for: Blocking

製品の詳細

特性

Our Abpromise guarantee covers the use of ab4967 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • アプリケーション

    Blocking

  • 製品の状態

    Lyophilized

  • 備考


    This peptide may be used for neutralization and control experiments with the polyclonal antibody that reacts with this product and bovine rhodopsin, catalog ab3424. Using a solution of peptide of equal volume and concentration to the corresponding antibody will yield a large molar excess of peptide (~ 70-fold) for competitive inhibition of antibody-protein binding reactions.

  • Concentration information loading...

前処理および保存

  • 保存方法および安定性

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 再構成
    >95% pure, lyophilized synthetic peptide. Reconstitute with 0.1 ml of distilled water.

関連情報

  • 別名

    • CSNBAD1
    • MGC138309
    • MGC138311
    • OPN 2
    • OPN2
    • opsd
    • OPSD_HUMAN
    • opsin 2
    • Opsin 2 rod pigment
    • Opsin-2
    • Opsin2
    • Retinitis Pigmentosa 4
    • Retinitis pigmentosa 4 autosomal dominant
    • RHO
    • Rhodopsin
    • RP 4
    • RP4
    see all

  • 機能

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.

  • 組織特異性

    Rod shaped photoreceptor cells which mediates vision in dim light.

  • 関連疾患

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1

  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

  • 翻訳後修飾

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.

  • 細胞内局在

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Information by UniProt

プロトコール

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

参考文献 (1)

ab4967 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab4967 は 1 報の論文で使用されています。

  • Park YM  et al. Acute estradiol treatment reduces skeletal muscle protein breakdown markers in early- but not late-postmenopausal women. Steroids 146:43-49 (2019). PubMed: 30928279

レビューと Q&A

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Question

I am looking for a control to use with derivatized surface that captures lipid assemblies. I was looking into rhodopsin as a potential candidate. Do you know if this rhodopsin peptide contains enough of the opsin moiety that it will be captured in this lipid bilayer surface?



For further information, here is a link to the liposome capturing kit from Proteon that we will be using: http://www.bio-rad.com/webroot/web/pdf/lsr/literature/10024332A.pdf

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Answer

Thank you for contacting Abcam in regards to ab4967.
We have never tested this peptide in this particular application, so we cannot say whether it would work in this application or not.
I am sorry that I could not be more helpful in this matter but if there is anything else I can help you with, please let me know.

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