保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
バッファーPreservative: 0.09% Sodium Azide
Constituents: 50% Glycerol, PBS, pH 7.4
Concentration information loading...
精製度Protein G purified
Our Abpromise guarantee covers the use of ab98887 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Flow Cyt||Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|WB||1/1000. Predicted molecular weight: 39 kDa.|
|IP||Use at an assay dependent concentration. 1-2 µg per 100-500µg of protein.|
|ELISA||Use at an assay dependent concentration.|
|IHC-P||Use at an assay dependent concentration.|
|IHC-Fr||Use at an assay dependent concentration.|
|ICC||Use at an assay dependent concentration.|
機能Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
組織特異性Rod shaped photoreceptor cells which mediates vision in dim light.
関連疾患Retinitis pigmentosa 4
Night blindness, congenital stationary, autosomal dominant 1
配列類似性Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
翻訳後修飾Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
Contains one covalently linked retinal chromophore.
細胞内局在Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
- Information by UniProt
- CSNBAD1 antibody
- MGC138309 antibody
- MGC138311 antibody
Immunohistochemistry analysis staining Rhodopsin in mouse retina with ab98887 at 1:1000. Secondary Antibody: FITC Goat Anti-Mouse (green). Counterstain: DAPI (blue) nuclear stain.
Overlay histogram showing Y79 cells stained with ab98887 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab98887, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in Y79 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
This product has been referenced in:
- Albalawi A et al. Carnosic acid attenuates acrylamide-induced retinal toxicity in zebrafish embryos. Exp Eye Res 175:103-114 (2018). Read more (PubMed: 29928899) »
- Raghupathy RK et al. Rpgrip1 is required for rod outer segment development and ciliary protein trafficking in zebrafish. Sci Rep 7:16881 (2017). IHC-P ; Zebrafish . Read more (PubMed: 29203866) »