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  1. Link

    rhodopsin-antibody-1d4-ab5417.pdf

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Signal Transduction Signaling Pathway G Protein Signaling GPCR
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Anti-Rhodopsin 抗体 [1D4] (ab5417)

  • Datasheet
  • SDS
Reviews (4)Q&A (1)References (75)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

Key features and details

  • Mouse monoclonal [1D4] to Rhodopsin
  • Suitable for: ICC, ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-P
  • Reacts with: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
  • Isotype: IgG1

こちらの製品もご検討ください

一次抗体
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Anti-MLKL (phospho S358) antibody [EPR9514] (ab187091)
二次抗体
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)
一次抗体
Anti-Retinal S antigen antibody (ab3435)

関連製品

製品の概要

  • 製品名

    Anti-Rhodopsin antibody [1D4]
    Rhodopsin 一次抗体 製品一覧
  • 製品の詳細

    Mouse monoclonal [1D4] to Rhodopsin
  • 由来種

    Mouse
  • 特異性

    ab5417 detects Rhodopsin from human and bovine retinal samples. Data from Yin J et al., 2012 (PMID 22743318) indicates that in Zebrafish ab5417 appears to recognize Red Opsin rather than Rhodopsin.

  • アプリケーション

    適用あり: ICC, ELISA, IHC-FoFr, IP, WB, IHC-Fr, IHC-Pmore details
  • 種交差性

    交差種: Mouse, Rat, Cow, Human, Zebrafish, Amphibian
    交差が予測される動物種: Rabbit
  • 免疫原

    Tissue, cells or virus corresponding to Bovine Rhodopsin. Bleached bovine ROS [rod outer segment] disk membranes

  • エピトープ

    The epitope for this antibody has been localized to the C-terminal nine amino acids of bovine rhodopsin known as the 1D4 epitope.
  • ポジティブ・コントロール

    • WB: HL60 whole cell lysate IHC-P: Human and mouse retinal tissue
  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

  • 製品の状態

    Liquid
  • 保存方法

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • バッファー

    Preservative: 0.05% Sodium azide
    Constituents: 99% PBS, 0.1% BSA
  • Concentration information loading...
  • 精製度

    Protein G purified
  • 一次抗体 備考

    Vision involves the conversion of light into electrochemical signals that are processed by the retina and subsequently sent to and interpreted by the brain. The process of converting light to an electrochemical signal begins when the membrane-bound protein, rhodopsin, absorbs light within the retina. Photoexcitation of rhodopsin causes the cytoplasmic surface of the protein to become catalytically active. In the active state, rhodopsin activates transducin, a GTP binding protein. Once activated, transducin promotes the hydrolysis of cGMP by phosphodiesterase (PDE). The decrease of intracellular cGMP concentrations causes the ion channels within the outer segment of the rod or cone to close, thus causing membrane hyperpolarization and, eventually, signal transmission. Rhodopsin’s activity is believed to be shut off by its phosphorylation followed by binding of the soluble protein arrestin.
  • ポリ/モノ

    モノクローナル
  • クローン名

    1D4
  • アイソタイプ

    IgG1
  • 研究分野

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • GPCR
    • Neuroscience
    • Sensory System
    • Visual system

関連製品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5417の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
ICC
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19587120
IP
Use at an assay dependent concentration.
WB (2)
1/100 - 1/1000. Detects a band of approximately 40 kDa.
IHC-Fr (1)
Use at an assay dependent concentration. PubMed: 22743318
IHC-P
1/100 - 1/1000.
特記事項
ICC
Use at an assay dependent concentration.
ELISA
Use at an assay dependent concentration.
IHC-FoFr
Use at an assay dependent concentration. PubMed: 19587120
IP
Use at an assay dependent concentration.
WB
1/100 - 1/1000. Detects a band of approximately 40 kDa.
IHC-Fr
Use at an assay dependent concentration. PubMed: 22743318
IHC-P
1/100 - 1/1000.

ターゲット情報

  • 機能

    Photoreceptor required for image-forming vision at low light intensity. Required for photoreceptor cell viability after birth. Light-induced isomerization of 11-cis to all-trans retinal triggers a conformational change leading to G-protein activation and release of all-trans retinal.
  • 組織特異性

    Rod shaped photoreceptor cells which mediates vision in dim light.
  • 関連疾患

    Retinitis pigmentosa 4
    Night blindness, congenital stationary, autosomal dominant 1
  • 配列類似性

    Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.
  • 翻訳後修飾

    Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.
    Contains one covalently linked retinal chromophore.
  • 細胞内局在

    Membrane. Synthesized in the inner segment (IS) of rod photoreceptor cells before vectorial transport to the rod outer segment (OS) photosensory cilia.
  • Target information above from: UniProt accession P08100 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 参照データベース

    • Entrez Gene: 509933 Cow
    • Entrez Gene: 100307115 Human
    • Entrez Gene: 6010 Human
    • Entrez Gene: 212541 Mouse
    • Entrez Gene: 100009200 Rabbit
    • Entrez Gene: 24717 Rat
    • Entrez Gene: 30295 Zebrafish
    • Omim: 180380 Human
    • SwissProt: P02699 Cow
    • SwissProt: P08100 Human
    • SwissProt: P15409 Mouse
    • SwissProt: P49912 Rabbit
    • SwissProt: P51489 Rat
    • SwissProt: P35359 Zebrafish
    • Unigene: 247565 Human
    • Unigene: 2965 Mouse
    • Unigene: 406156 Mouse
    • Unigene: 92530 Rat
    • Unigene: 354 Zebrafish
    see all
  • 別名

    • CSNBAD1 antibody
    • MGC138309 antibody
    • MGC138311 antibody
    • OPN 2 antibody
    • OPN2 antibody
    • opsd antibody
    • OPSD_HUMAN antibody
    • opsin 2 antibody
    • Opsin 2 rod pigment antibody
    • Opsin-2 antibody
    • Opsin2 antibody
    • Retinitis Pigmentosa 4 antibody
    • Retinitis pigmentosa 4 autosomal dominant antibody
    • RHO antibody
    • Rhodopsin antibody
    • RP 4 antibody
    • RP4 antibody
    see all

画像

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)Pearring et al PLoS Genet. 2017 Apr 14;13(4):e1006740. doi: 10.1371/journal.pgen.1006740. eCollection 2017 Apr. Fig 3. Reproduced under the Creative Commons license http://creativecommons.org/licenses/by/4.0/

    Deletion of Arf4 mice from the retina does not disrupt rhodopsin localization or photoreceptor morphology.

    E. Arf4 immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Image of the photoreceptor IS where the biosynthetic membranes are localized. Eyes were collected at P34. Scale bar = 10 μm.

    F. Rhodopsin immunostaining in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P34. Scale bar = 10 μm.

    G. Comparative analysis of photoreceptor morphology in Arf4flox/CagCreER experimental and control retinal cross-sections. Eyes were collected at P41. Scale bar = 20 μm.

    OS = outer segment, IS = inner segment, ONL = outer nuclear layer, OPL = outer plexiform layer.

  • Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
    Western blot - Anti-Rhodopsin antibody [1D4] (ab5417)
    Anti-Rhodopsin antibody [1D4] (ab5417) at 1/500 dilution + HL60 (Human promyelocytic leukemia cell line) cell lysate at 25 µg

    Observed band size: 40 kDa why is the actual band size different from the predicted?

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of formalin-fixed mouse retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

    A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

    The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of formalin-fixed human retinal tissue, labeling rhodopsin with ab5417 at a 1:50 dilution in 3% BSA-PBS solution and incubated at 4°C overnight in a high humidity environment.

    A DyLight® 488 secondary antibody was used (green) incubated at room temperature in the dark. The tissue was counterstained with DAPI against DNA, showing nuclear compartments. Prior to staining the formalin-fixed tissue was permeabilized with 0.1% Triton X-100 in TBS for between 5 and 10 minutes, then blocked with 3% BSA-PBS for 30 minutes at room temperature.

    The left image is a negative control with only the secondary antibody and the right image is in the presence of ab5417 and the secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of paraffin-embedded mouse retinal tissue labeling Rhodopsin with ab5417.

    Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrival using 10mM sodium citrate at pH 6.0, then the sample was microwaved for 8 to 15 minutes. Subsequent to retrival the retinal tissue was blocked for 15 minutes at room temperature with 3% hydrogen peroxide. The sample was then incubated with ab5417 in 3% BSA-PBS at 4°C at a dilution of 1:1000, overnight. Hematoxylin was used to counterstain the tissue.

    The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Rhodopsin antibody [1D4] (ab5417)

    Immunohistochemical analysis of paraffin-embedded human retinal tissue labeling Rhodopsin with ab5417.

    Secondary used was HRP conjugated. Prior preparation was initiated by antigen retrieval was performed using 10mM sodium citrate (pH 6.0), microwaved for 8-15 min. Subsequent to retrival the retinal tissue was blocked in 3% H2O2-methanol for 15 min at room temperature. The sample was then incubated with ab5417 in 3% BSA-PBS at a dilution of 1:200 overnight at 4°C, overnight. Hematoxylin was used to counterstain the tissue.

    The left side of the image is shown as a negative control and is the tissue in the absence of ab5417, the right side is in the prescence of the counterstain, ab5417 and the HRP conjugated secondary.

プロトコール

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

データシートおよび資料

  • SDS download

  • Datasheet download

    Download

参考文献 (75)

ab5417 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5417 は 75 報の論文で使用されています。

  • Rodgers J  et al. Using a bistable animal opsin for switchable and scalable optogenetic inhibition of neurons. EMBO Rep 22:e51866 (2021). PubMed: 33655694
  • Yi MH  et al. Optogenetic activation of spinal microglia triggers chronic pain in mice. PLoS Biol 19:e3001154 (2021). PubMed: 33739978
  • Toulis V  et al. The Deubiquitinating Enzyme Ataxin-3 Regulates Ciliogenesis and Phagocytosis in the Retina. Cell Rep 33:108360 (2020). PubMed: 33176149
  • Cheng J  et al. Interferon Alpha Induces Multiple Cellular Proteins That Coordinately Suppress Hepadnaviral Covalently Closed Circular DNA Transcription. J Virol 94:N/A (2020). PubMed: 32581092
  • Emam A  et al. Retinal morphology in Astyanax mexicanus during eye degeneration. J Comp Neurol 528:1523-1534 (2020). PubMed: 31811648
View all Publications for this product

レビューと Q&A

Show All レビュー Q&A
レビューを送る 質問を送る

1-5 of 5 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-Rhodopsin antibody [1D4]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Retina)
Permeabilization
Yes - 20-minute incubation 0,5% Triton X-100 in 1x PBS and 2-minute incubation in 0.05mg/ml Proteinase K in 1x PBS (both at RT)
Specification
Retina
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Dec 07 2021

Western blot abreview for Anti-Rhodopsin antibody [1D4]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Arabidopsis thaliana Cell lysate - whole cell (vegetative lead tissue)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE gel)
Loading amount
100 µg
Specification
vegetative lead tissue
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

P Ding

Verified customer

投稿 Aug 11 2021

Western blot abreview for Anti-Rhodopsin [1D4] antibody

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Loading amount
10 µg
Gel Running Conditions
Non-reduced Denaturing (12%)
Sample
Human Cell lysate - whole cell (neuroblast)
Specification
neuroblast
Blocking step
BSA as blocking agent for 50 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

投稿 Jun 17 2014

Immunohistochemistry (Frozen sections) abreview for Anti-Rhodopsin [1D4] antibody

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 22°C
Sample
Zebrafish Tissue sections (Long double cone (red cone) photoreceptor cells)
Specification
Long double cone (red cone) photoreceptor cells
Permeabilization
Yes - PBST
Fixative
Paraformaldehyde
Read More
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Dr. Jun Yin

Verified customer

投稿 Jul 02 2013

Question

Would like to try this antibody in IP.

Read More

Abcam community

Verified customer

Asked on Jul 16 2012

Answer

Thank you very much for your call today. It was a pleasure speaking to you. I've created the discount code below, and if you have any further questions about redeeming the code or submitting the Abreview, please let me know and I'll be happy to help.

DISCOUNT CODE: 10***
Expiration date: November 17, 2012

This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for ab5417 in IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active. For more information on how to submit an Abreview, please visit the site: https://www.abcam.com/Abreviews.

Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More

Abcam Scientific Support

Answered on Jul 16 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

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