Anti-RGS4 抗体 (ab97307)
Key features and details
- Rabbit polyclonal to RGS4
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-RGS4 antibody
RGS4 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RGS4 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-198 of Human RGS4 (AAH00737)
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ポジティブ・コントロール
- WB: Raji cell lysate, rat and mouse brain tissue extract; IHC-P: Human breast cancer and mouse muscle tissue.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab97307の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P |
1/100 - 1/1000.
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WB |
1/500 - 1/3000. Predicted molecular weight: 23 kDa.
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特記事項 |
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IHC-P
1/100 - 1/1000. |
WB
1/500 - 1/3000. Predicted molecular weight: 23 kDa. |
ターゲット情報
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機能
Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Activity on G(z)-alpha is inhibited by phosphorylation of the G-protein. Activity on G(z)-alpha and G(i)-alpha-1 is inhibited by palmitoylation of the G-protein. -
関連疾患
Genetic variation in RGS4 is associated with susceptibility to schizophrenia type 9 (SCZD9) [MIM:604906]. A complex, multifactorial psychotic disorder or group of disorders characterized by disturbances in the form and content of thought (e.g. delusions, hallucinations), in mood (e.g. inappropriate affect), in sense of self and relationship to the external world (e.g. loss of ego boundaries, withdrawal), and in behavior (e.g bizarre or apparently purposeless behavior). Although it affects emotions, it is distinguished from mood disorders in which such disturbances are primary. Similarly, there may be mild impairment of cognitive function, and it is distinguished from the dementias in which disturbed cognitive function is considered primary. Some patients manifest schizophrenic as well as bipolar disorder symptoms and are often given the diagnosis of schizoaffective disorder. -
配列類似性
Contains 1 RGS domain. -
翻訳後修飾
Palmitoylated on Cys-2 and/or Cys-12.
Phosphorylated by cyclic GMP-dependent protein kinase. - Information by UniProt
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参照データベース
- Entrez Gene: 5999 Human
- Entrez Gene: 19736 Mouse
- Entrez Gene: 29480 Rat
- Omim: 602516 Human
- SwissProt: P49798 Human
- SwissProt: O08899 Mouse
- SwissProt: P49799 Rat
- Unigene: 386726 Human
see all -
別名
- MGC2124 antibody
- MGC60244 antibody
- Regulator of G protein signalling 4 antibody
see all
画像
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Immunohistochemical analysis of paraffin-embedded human breast cancer tissue staining RGS4 with ab97307 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Raji (human Burkitt's lymphoma cell line) whole cell lysate at 30 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa
12% SDS-PAGE -
Immunohistochemical analysis of paraffin-embedded mouse muscle tissue staining RGS4 with ab97307 at 1:500 dilution.
Antigen Retrieval: EDTA based buffer, pH 8.0, 15min. -
Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Mouse cerebellum tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
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Anti-RGS4 antibody (ab97307) at 1/1000 dilution + Rat brain tissue extract at 50 µg
Secondary
HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 23 kDa12% SDS-PAGE
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (1)
ab97307 は 1 報の論文で使用されています。
- Xiao D & Gao HX Mechanism of miR-107-targeting of regulator of G-protein signaling 4 in hepatocellular carcinoma. Oncol Lett 18:5145-5154 (2019). PubMed: 31612026