製品の概要
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製品名Anti-RGS3 antibody
RGS3 一次抗体 製品一覧 -
製品の詳細Rabbit polyclonal to RGS3
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由来種Rabbit
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アプリケーション適用あり: IP, WBmore details
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種交差性交差種: Human
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免疫原
E. coli expressed human RGS 3 isoform 1 (amino acids 401-519).
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特記事項
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
製品の特性
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製品の状態Liquid
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保存方法Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
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バッファーPBS pH7.2 with 50% glycerol, 1% BSA and 0.02% sodium azide
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Concentration information loading... -
精製度Immunogen affinity purified
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一次抗体 備考RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
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ポリ/モノポリクローナル
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アイソタイプIgG
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研究分野
関連製品
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Compatible Secondaries
アプリケーション
Our Abpromise guarantee covers the use of ab2564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| IP | Use at an assay dependent concentration. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa). |
ターゲット情報
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機能Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases.
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配列類似性Contains 1 C2 domain.
Contains 1 PDZ (DHR) domain.
Contains 1 RGS domain. -
翻訳後修飾Phosphorylated by cyclic GMP-dependent protein kinase.
ISGylated. -
細胞内局在Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear.
- Information by UniProt
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参照データベース
- Entrez Gene: 5998 Human
- Omim: 602189 Human
- SwissProt: P49796 Human
- Unigene: 494875 Human
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別名
- C2PA antibody
- FLJ20370 antibody
- FLJ31516 antibody
see all
画像
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Western blot - Anti-RGS3 antibody (ab2564)Lane 1 : Anti-RGS3 antibody (ab2564)
Lane 2 : Anti-RGS3 antibody (ab2564) at 1 µg/ml
Lane 1 : mock
Lane 2 : transfected HEK293 cells (right lane)
Predicted band size: 132 kDa
Observed band size: 70 kDa (why is the actual band size different from the predicted?)
参考文献
ab2564 has not yet been referenced specifically in any publications.
レビューと Q&A
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"