Anti-RGS3 抗体 (ab2564)
Key features and details
- Rabbit polyclonal to RGS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RGS3 antibody
RGS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RGS3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
E. coli expressed human RGS 3 isoform 1 (amino acids 401-519).
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特記事項
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
一次抗体 備考
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
Our Abpromise guarantee covers the use of ab2564 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
アプリケーション | Abreviews | 特記事項 |
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WB | Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa). |
ターゲット情報
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機能
Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases. -
配列類似性
Contains 1 C2 domain.
Contains 1 PDZ (DHR) domain.
Contains 1 RGS domain. -
翻訳後修飾
Phosphorylated by cyclic GMP-dependent protein kinase.
ISGylated. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 5998 Human
- Omim: 602189 Human
- SwissProt: P49796 Human
- Unigene: 494875 Human
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別名
- C2PA antibody
- FLJ20370 antibody
- FLJ31516 antibody
see all
画像
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Lane 1 : Anti-RGS3 antibody (ab2564)
Lane 2 : Anti-RGS3 antibody (ab2564) at 1 µg/ml
Lane 1 : mock
Lane 2 : transfected HEK293 cells (right lane)
Predicted band size: 132 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
データシートおよび資料
参考文献 (0)
ab2564 は論文での使用が確認できていません。