Anti-RGS3 抗体 (ab2564)
Key features and details
- Rabbit polyclonal to RGS3
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-RGS3 antibody
RGS3 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to RGS3 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
E. coli expressed human RGS 3 isoform 1 (amino acids 401-519).
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called 3280 RGS3 Antibody.
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 1% BSA -
Concentration information loading... -
精製度
Immunogen affinity purified -
一次抗体 備考
RGS 3 (Regulator of G protein Signaling 3) is widely expressed in many tissues and cells. The protein contains an RGS domain near the carboxy terminus that serves as a GTPase activating protein (GAP). RGS 3 negatively regulates the activation of Gialpha and G alpha q. A truncated RGS 3 (RGS 3T), with its N-terminal 313 residues removed, has been shown to negatively regulate acenylyl cyclase and PLC. RGS 3T may be localized in the nucleus and RGS 3 is found in cytoplasm. Translocation to the membrane is important for the RGS proteins to interact with heterotrimeric G proteins. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab2564の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa).
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| 特記事項 |
|---|
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 132 kDa). |
ターゲット情報
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機能
Down-regulates signaling from heterotrimeric G-proteins by increasing the GTPase activity of the alpha subunits, thereby driving them into their inactive GDP-bound form. Down-regulates G-protein-mediated release of inositol phosphates and activation of MAP kinases. -
配列類似性
Contains 1 C2 domain.
Contains 1 PDZ (DHR) domain.
Contains 1 RGS domain. -
翻訳後修飾
Phosphorylated by cyclic GMP-dependent protein kinase.
ISGylated. -
細胞内局在
Cytoplasm. Nucleus. Cell membrane. Long isoforms are cytoplasmic and associated with the plasma membrane. Short isoforms are nuclear. - Information by UniProt
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参照データベース
- Entrez Gene: 5998 Human
- Omim: 602189 Human
- SwissProt: P49796 Human
- Unigene: 494875 Human
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別名
- C2PA antibody
- FLJ20370 antibody
- FLJ31516 antibody
see all
画像
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Western blot - Anti-RGS3 antibody (ab2564)Lane 1 : Anti-RGS3 antibody (ab2564)
Lane 2 : Anti-RGS3 antibody (ab2564) at 1 µg/ml
Lane 1 : mock
Lane 2 : transfected HEK293 cells (right lane)
Predicted band size: 132 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab2564 は論文での使用が確認できていません。