Anti-RFXANK 抗体 [OTI3E7] (ab236408)
Key features and details
- Mouse monoclonal [OTI3E7] to RFXANK
- Suitable for: WB, ICC/IF, IHC-P, Flow Cyt (Intra)
- Reacts with: Human
- Isotype: IgG2b
製品の概要
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製品名
Anti-RFXANK antibody [OTI3E7] -
製品の詳細
Mouse monoclonal [OTI3E7] to RFXANK -
由来種
Mouse -
アプリケーション
適用あり: WB, ICC/IF, IHC-P, Flow Cyt (Intra)more details -
種交差性
交差種: Human -
免疫原
Recombinant full length protein corresponding to Human RFXANK aa 1 to the C-terminus. Produced in HEK293T cells. NP_604389
Database link: NP_604389 -
ポジティブ・コントロール
- WB: pCMV6-ENTRY RFXANK cDNA transfected HEK-293T cell lysate. IHC-P: Human kidney, kidney carcinoma, ovary adenocarcinoma, pancreas, endometrium adenocarcinoma, bladder carcinoma and tonsil tissues. ICC/IF: COS-7 cells transiently transfected by pCMV6-ENTRY RFXANK. Flow Cyt (Intra): HeLa and Jurkat cells.
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特記事項
Clone OTI3E7 (formerly 3E7).
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 1% BSA, 50% Glycerol -
Concentration information loading...
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精製度
Affinity purified -
特記事項(精製)
Purified from cell culture supernatant. -
ポリ/モノ
モノクローナル -
クローン名
OTI3E7 -
アイソタイプ
IgG2b -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab236408の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000. Predicted molecular weight: 24, 28 kDa.
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ICC/IF |
1/100.
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IHC-P |
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Flow Cyt (Intra) |
1/100.
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特記事項 |
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WB
1/2000. Predicted molecular weight: 24, 28 kDa. |
ICC/IF
1/100. |
IHC-P
1/150. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Flow Cyt (Intra)
1/100. |
ターゲット情報
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機能
Activates transcription from class II MHC promoters. Activation requires the activity of the MHC class II transactivator (MHC2TA). May regulate other genes in the cell. RFX binds the X1 box of MHC-II promoters. Isoform RFX-B-delta5 is not involved in the positive regulation of MHC class II genes. -
組織特異性
Ubiquitous. -
関連疾患
Defects in RFXANK are a cause of bare lymphocyte syndrome type 2 (BLS2) [MIM:209920]; also known as hereditary MHC class II deficiency or HLA class II-deficient combined immunodeficiency. BLS2 is a severe combined immunodeficiency disease with early onset. It is characterized by a profound defect in constitutive and interferon-gamma induced MHC II expression, absence of cellular and humoral T-cell response to antigen challenge, hypogammaglobulinemia and impaired antibody production. The consequence include extreme susceptibility to viral, bacterial and fungal infections. -
配列類似性
Contains 5 ANK repeats. -
ドメイン
The third ankyrin repeat is required for association with the two other RFX subunits; RFX5 and RFXAP. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 8625 Human
- Omim: 603200 Human
- SwissProt: O14593 Human
- Unigene: 153629 Human
- Unigene: 296776 Human
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別名
- ANKRA1 antibody
- Ankyrin repeat containing regulatory factor X associated protein antibody
- Ankyrin repeat family A protein 1 antibody
see all
画像
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All lanes : Anti-RFXANK antibody [OTI3E7] (ab236408) at 1/2000 dilution
Lane 1 : pCMV6-ENTRY control cDNA transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
Lane 2 : pCMV6-ENTRY RFXANK cDNA transfected HEK-293T cell lysate
Lysates/proteins at 5 µg per lane.
Predicted band size: 24, 28 kDa -
Paraffin-embedded human kidney tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human kidney carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human ovary adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human pancreas tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human endometrium adenocarcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human bladder carcinoma tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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Paraffin-embedded human tonsil tissue stained for RFXANK using ab236408 at 1/150 dilution in immunohistochemical analysis.
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COS-7 (african green monkey kidney fibroblast-like cell line) cells transiently transfected by pCMV6-ENTRY RFXANK stained for RFXANK (green) using ab236408 at 1/100 dilution in ICC/IF.
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Flow cytometric analysis of HeLa (human epithelial cell line from cervix adenocarcinoma) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
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Flow cytometric analysis of Jurkat (human T cell leukemia cell line from peripheral blood) cell line labeling RFXANK with ab226408 at 1/100 dilution (red) compared to a nonspecific negative control antibody (blue).
プロトコール
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab236408 は論文での使用が確認できていません。