Anti-RFP 抗体 [RF5R] (ab125244)
Key features and details
- Mouse monoclonal [RF5R] to RFP
- Suitable for: WB
- Reacts with: Species independent
- Isotype: IgG1
製品の概要
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製品名
Anti-RFP antibody [RF5R]
RFP 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [RF5R] to RFP -
由来種
Mouse -
特異性
Recognizes native and denatured forms of RFP and its turbo-RFP, DsRed variants
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アプリケーション
適用あり: WBmore details -
種交差性
交差種: Species independent -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Transfected HEK293 cell lysate.
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特記事項
Store at 4°C (add 0.1% NaN3) for several days to weeks.
This product was changed from ascites to tissue culture supernatant on 5th February 2018. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading...
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精製度
Protein A purified -
特記事項(精製)
ab125244 is purified by protein A affinity chromatography from TCS -
ポリ/モノ
モノクローナル -
クローン名
RF5R -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab125244の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/1000 - 1/3000. Predicted molecular weight: 26 kDa.
Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins. |
特記事項 |
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WB
1/1000 - 1/3000. Predicted molecular weight: 26 kDa. Sensitivity with ECL: 1-10ng of purified RFP or RFP fusion proteins. |
ターゲット情報
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関連性
Fluorescent proteins have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions. -
別名
- DsRed antibody
- GFP like chromoprotein antibody
- red fluorescent protein antibody
- RFP antibody
画像
プロトコール
データシートおよび資料
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Datasheet download
参考文献 (11)
ab125244 は 11 報の論文で使用されています。
- Su M et al. Loss of Calretinin in L5a impairs the formation of the barrel cortex leading to abnormal whisker-mediated behaviors. Mol Brain 14:67 (2021). PubMed: 33845857
- Pereira Luppi M et al. Sox6 expression distinguishes dorsally and ventrally biased dopamine neurons in the substantia nigra with distinctive properties and embryonic origins. Cell Rep 37:109975 (2021). PubMed: 34758317
- Leung C et al. Lgr5 Marks Adult Progenitor Cells Contributing to Skeletal Muscle Regeneration and Sarcoma Formation. Cell Rep 33:108535 (2020). PubMed: 33357435
- Kueck T et al. Vesicular Stomatitis Virus Transcription Is Inhibited by TRIM69 in the Interferon-Induced Antiviral State. J Virol 93:N/A (2019). PubMed: 31578292
- Seishima R et al. Neonatal Wnt-dependent Lgr5 positive stem cells are essential for uterine gland development. Nat Commun 10:5378 (2019). PubMed: 31772170