Anti-RFC 抗体 (ab272354)

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Western blot - Anti-RFC antibody (ab272354)

    Key features and details

    • Rabbit polyclonal to RFC
    • Suitable for: WB
    • Reacts with: Mouse, Rat, Human, African green monkey
    • Isotype: IgG

    製品の概要

    • 製品名

      Anti-RFC antibody

    • 製品の詳細

      Rabbit polyclonal to RFC

    • 由来種

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Mouse
      Rat
      Human
      African green monkey
      See all applications and species data

    • 免疫原

      Synthetic peptide corresponding to Human RFC aa 200-300.
      Database link: P41440

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • ポジティブ･コントロール

      • WB: Rat brain and ovary tissue lysates; mouse brain, testis and ovary lysates; human placenta tissue lysate; HEK-293, PC-3 , 22RV1, K562, NIH/3T3 and COS-7 whole cell lysates.

    • 特記事項

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    製品の特性

    アプリケーション

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab272354 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    アプリケーション Species
    WB
    Mouse
    Rat
    Human
    African green monkey
    アプリケーション Abreviews 特記事項
    WB
    Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa.
    特記事項
    WB
    Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 65 kDa.

    ターゲット情報

    • 機能

      Transporter for the intake of folate. Uptake of folate in human placental choriocarcinoma cells occurs by a novel mechanism called potocytosis which functionally couples three components, namely the folate receptor, the folate transporter, and a V-type H(+)-pump.

    • 組織特異性

      Placenta, liver, and to a much smaller extent, in lung.

    • 配列類似性

      Belongs to the reduced folate carrier (RFC) transporter (TC 2.A.48) family.

    • 翻訳後修飾

      Heavily glycosylated.

    • 細胞内局在

      Membrane.
    • Information by UniProt

    • 参照データベース

      see all

    • 別名

      • CHMD antibody
      • FLOT 1 antibody
      • FLOT1 antibody
      • Folate transporter 1 antibody
      • FOLT antibody
      • IFC 1 antibody
      • IFC-1 antibody
      • IFC1 antibody
      • Intestinal folate carrier 1 antibody
      • Intestinal folate carrier antibody
      • OTTHUMP00000115459 antibody
      • OTTHUMP00000115460 antibody
      • Placental folate transporter antibody
      • Reduced folate carrier antibody
      • Reduced folate carrier protein antibody
      • REFC antibody
      • RFC 1 antibody
      • RFC antibody
      • RFC1 antibody
      • S19A1_HUMAN antibody
      • SLC19A1 antibody
      • Solute carrier family 19 member 1 antibody
      see all

    画像

    • Western blot - Anti-RFC antibody (ab272354)
      Western blot - Anti-RFC antibody (ab272354)
      All lanes : Anti-RFC antibody (ab272354) at 0.5 µg/ml

      Lane 1 : Rat brain tissue lysates
      Lane 2 : Rat ovary tissue lysates
      Lane 3 : Mouse brain tissue lysates
      Lane 4 : Mouse testicular tissue lysates
      Lane 5 : Mouse ovary tissue lysates
      Lane 6 : NIH/3T3 (mouse embryo fibroblast cell line) whole cell lysates
      Lane 7 : Human placenta tissue lysates
      Lane 8 : HEK-293 (human epithelial cell line from embryonic kidney) whole cell lysates
      Lane 9 : COS-7 (African green monkey kidney fibroblast-like cell line) whole cell lysates
      Lane 10 : PC-3 (human prostate adenocarcinoma cell line) whole cell lysates
      Lane 11 : 22RV1 (human prostate carcinoma cell line) whole cell lysates
      Lane 12 : K562 (human chronic myelogenous leukemia cell line from bone marrow) whole cell lysates

      Lysates/proteins at 50 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit IgG-HRP at 1/10000 dilution

      Developed using the ECL technique.

      Predicted band size: 65 kDa
      Observed band size: 65 kDa



      5-20% SDS-PAGE gel.

      Blocked membrane with 5% Non-fat Milk/ TBS for 1.5 hour at room temperature.

    プロトコール

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    参考文献 (0)

    ab272354 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

    ab272354 は論文での使用が確認できていません。

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