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Anti-Retinoic Acid Receptor gamma 1 抗体 (ab5904)

Submit a review Q&A (1)References (2)
Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
  • Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
  • Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)

Key features and details

  • Rabbit polyclonal to Retinoic Acid Receptor gamma 1
  • Suitable for: WB
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

製品の概要

  • 製品名

    Anti-Retinoic Acid Receptor gamma 1 antibody

  • 製品の詳細

    Rabbit polyclonal to Retinoic Acid Receptor gamma 1

  • 由来種

    Rabbit

  • アプリケーション

    適用あり: WBmore details

  • 種交差性

    交差種: Mouse, Rat, Human
    交差が予測される動物種: Rabbit

  • 免疫原

    Synthetic peptide corresponding to Retinoic Acid Receptor gamma 1 aa 40-57 (N terminal).
    Sequence:

    EMLSPSFRGLGQPDLPKE


    (Peptide available as ab39773)
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

  • ポジティブ･コントロール

    • WB: MCF7, BeWo, HaCaT, HepG2 and HEK293 cell lysate. Mouse Mammary Gland and Rat brain tissue lysate.

  • 特記事項

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

製品の特性

アプリケーション

The Abpromise guarantee

Abpromise保証は、 次のテスト済みアプリケーションにおけるab5904の使用に適用されます

アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。

アプリケーション Abreviews 特記事項
WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).
特記事項
WB
1/2000. Detects a band of approximately 50 kDa (predicted molecular weight: 54 kDa).

ターゲット情報

  • 機能

    Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RAR/RXR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence of ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. Required for limb bud development. In concert with RARA or RARB, required for skeletal growth, matrix homeostasis and growth plate function.

  • 配列類似性

    Belongs to the nuclear hormone receptor family. NR1 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.

  • ドメイン

    Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain.

  • 細胞内局在

    Nucleus.
  • Information by UniProt

  • 参照データベース

    see all

  • 別名

    • NR1B3 antibody
    • Nuclear receptor subfamily 1 group B member 3 antibody
    • RAR gamma antibody
    • RAR gamma isoform 1 antibody
    • RAR-gamma antibody
    • RARC antibody
    • Rarg antibody
    • RARG_HUMAN antibody
    • Retinoic acid receptor gamma antibody
    see all

画像

  • Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
    Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
    All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/500 dilution

    Lane 1 : MCF7 Nuclear enriched extract lysate
    Lane 2 : BeWo Nuclear enriched extract lysate
    Lane 3 : HaCaT Nuclear enriched extract lysate
    Lane 4 : HepG2 Nuclear enriched extract lysate
    Lane 5 : Mouse Mammary Gland tissue lysate

    Lysates/proteins at 50 µg per lane.

    Secondary
    All lanes : Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution

    Predicted band size: 54 kDa
    Observed band size: 60 kDa why is the actual band size different from the predicted?



    Detection: chemiluminescence.

     

  • Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
    Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
    All lanes : Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)

    Lane 1 : HEK293 cell lysate with neutralizing peptide
    Lane 2 : HEK293 cell lysate

    Predicted band size: 54 kDa

  • Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
    Western blot - Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904)
    Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) at 1/250 dilution + Rat brain tissue lysate at 30 µg

    Secondary
    Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate at 0.25 µg/ml

    Predicted band size: 54 kDa
    Observed band size: 42 kDa why is the actual band size different from the predicted?



    Detection: chemiluminescence.

     

参考文献 (2)

ab5904 を使用した論文を発表された方は、こちらまでお知らせください。データシートに掲載させていただきます。

ab5904 は 2 報の論文で使用されています。

  • Kadigamuwa C  et al. Role of Retinoic Acid Receptor-? in DNA Damage-Induced Necroptosis. iScience 17:74-86 (2019). PubMed: 31255985
  • Joss-Moore L  et al. Intrauterine growth restriction transiently delays alveolar formation and disrupts retinoic acid receptor expression in the lung of female rat pups. Pediatr Res 73:612-20 (2013). PubMed: 23419538

レビューと Q&A

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Question

Inquiry: Hi Sir, please tell about the quantity of Anti-Retinoic Acid Receptor gamma 1 antibody (ab5904) product in ug. as its not given there in the product information...! looking for a prompt reply as i have to do ChIP tomorrow. Thanks in advance.

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Answer

Thank you for your enquiry.

I can confirm that ab5904 Retinoic Acid Receptor gamma 1 antibody is sold as whole antiserum.

Unpurified antibodies, such as those sold as whole antiserum, ascites or tissue culture supernatant will not have a concentration stated on the datasheet. Antibody concentration is usually determined by protein assay, and serum / ascites / tissue culture supernatant will contain a lot of other proteins, which means the antibody quantification would not be accurate.

I can confirm that for whole antiserum, concentration of antibody is known to very between 1 - 10 mg/ml.


I also would like to make you aware that ab5904 is not tested in CHIP and therefore is not covered by our guarantee for CHIP.
We wouldlike to receive feedback on how this antibody performed in your CHIP experiment.


I am sorry we are not able to provide an exact concentration on this occasion, but hope this information will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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