Anti-Retinoic Acid Receptor beta 抗体 (ab5792)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor beta
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Retinoic Acid Receptor beta antibody
Retinoic Acid Receptor beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Retinoic Acid Receptor beta -
由来種
Rabbit -
特異性
This antibody shows slight cross-reactivity to RAR alpha but does not detect RAR gamma. -
アプリケーション
適用あり: ICC/IF, WBmore details -
種交差性
交差種: Mouse, Human -
免疫原
Synthetic peptide corresponding to Mouse Retinoic Acid Receptor beta aa 429-448.
Sequence:PSUSPSSVENSGVSQSPLLQ
(Peptide available asab5897) -
ポジティブ･コントロール
- WB: SH-SY5Y cell extract ; HEK-293 ICC/IF: SH-SY5Y
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
Preservative: 0.05% Sodium azide
Constituent: 99% PBS -
Concentration information loading... -
精製度
Whole antiserum -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab5792の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
| アプリケーション | Abreviews | 特記事項 |
|---|---|---|
| ICC/IF |
1/100.
|
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| WB |
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa).
|
| 特記事項 |
|---|
|
ICC/IF
1/100. |
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WB
1/1000. Detects a band of approximately 52 kDa (predicted molecular weight: 53 kDa). |
ターゲット情報
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機能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. -
関連疾患
Microphthalmia, syndromic, 12 -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
細胞内局在
Cytoplasm and Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5915 Human
- Entrez Gene: 218772 Mouse
- Omim: 180220 Human
- SwissProt: P10826 Human
- SwissProt: P22605 Mouse
- Unigene: 543218 Human
- Unigene: 581530 Human
- Unigene: 654490 Human
see all -
別名
- HAP antibody
- HBV-activated protein antibody
- NR1B2 antibody
see all
画像
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Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1 µg/ml
Lane 1 : SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate
Lane 2 : HEK-293 (Human epithelial cell line from embryonic kidney) whole cell lysate
Lane 3 : PANC-1 (Human pancreatic epithelial cancinoma cell line) whole cell lysate
Lane 4 : OVCAR-3 (Human ovary adenocarcinoma cell line) whole cell lysate
Lane 5 : BeWo (human placenta choriocarcinoma cell line) whole cell lysate
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : Goat anti-Rabbit IgG (H+L), Superclonal™ Recombinant Secondary Antibody, HRP at 1/4000 dilution
Predicted band size: 53 kDa
Additional bands at: ~58.50 kDa. We are unsure as to the identity of these extra bands.Detection: chemiluminescence
Western blot demonstrating antibody specificity by detection of differential basal expression of the target across cell lines owing to their inherent genetic constitution. The expression was observed in SH-SY5Y and HEK-293 and not seen in PANC-1, OVCAR-3 and BeWo using ab5792.
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Western blot - Anti-Retinoic Acid Receptor beta antibody (ab5792)All lanes : Anti-Retinoic Acid Receptor beta antibody (ab5792) at 1/1000 dilution
Lane 1 : SH-SY5Y cell lysate
Lane 2 : HepG2 cell lysate
Lane 3 : Mouse heart cell lysate
Lysates/proteins at 25 µg per lane.
Predicted band size: 53 kDa
Observed band size: 58 kDa why is the actual band size different from the predicted? -
Immunocytochemistry/ Immunofluorescence - Anti-Retinoic Acid Receptor beta antibody (ab5792)Immunofluorescent analysis of SH-SY5Y (Human neuroblastoma cell line from bone marrow) whole cell lysate cells on 70% confluent log phase labeling Retinoic Acid Receptor beta. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 2% BSA for 10 minutes at room temperature. The cells were labeled with ab5792 at 1/100 dilution in 0.1% BSA and incubated overnight at 4°C and then labeled with Goat anti-Rabbit IgG (H+L) secondary antibody, Alexa Fluor® 488 conjugate at 1/2000 dilution for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with DAPI. F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (1/300 dilution). Panel d is a merged image showing nuclear and cytoplasmic localization. Panel e represents BeWo (human placenta choriocarcinoma cell line) having no expression of Retinoic Acid Receptor beta. The images were captured at 60X magnification.
データシートおよび資料
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Datasheet download
参考文献 (0)
ab5792 は論文での使用が確認できていません。
