Anti-Retinoic Acid Receptor beta 抗体 (ab53161)
Key features and details
- Rabbit polyclonal to Retinoic Acid Receptor beta
- Suitable for: IP, ELISA, WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
製品の概要
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製品名
Anti-Retinoic Acid Receptor beta antibody
Retinoic Acid Receptor beta 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to Retinoic Acid Receptor beta -
由来種
Rabbit -
アプリケーション
適用あり: IP, ELISA, WB, IHC-P, ICC/IFmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide, corresponding to amino acids 351-390 of Human Retinoic Acid Receptor beta
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 0.87% Sodium chloride, 50% Glycerol (glycerin, glycerine), PBS -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53161の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IP |
Use at an assay dependent concentration. PubMed: 20655472
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ELISA |
Use at an assay dependent concentration.
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WB |
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa).
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IHC-P |
Use at an assay dependent concentration.
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ICC/IF |
Use a concentration of 1 - 5 µg/ml.
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特記事項 |
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IP
Use at an assay dependent concentration. PubMed: 20655472 |
ELISA
Use at an assay dependent concentration. |
WB
1/500 - 1/1000. Detects a band of approximately 50 kDa (predicted molecular weight: 50 kDa). |
IHC-P
Use at an assay dependent concentration. |
ICC/IF
Use a concentration of 1 - 5 µg/ml. |
ターゲット情報
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機能
Receptor for retinoic acid. Retinoic acid receptors bind as heterodimers to their target response elements in response to their ligands, all-trans or 9-cis retinoic acid, and regulate gene expression in various biological processes. The RXR/RAR heterodimers bind to the retinoic acid response elements (RARE) composed of tandem 5'-AGGTCA-3' sites known as DR1-DR5. In the absence or presence of hormone ligand, acts mainly as an activator of gene expression due to weak binding to corepressors. In concert with RARG, required for skeletal growth, matrix homeostasis and growth plate function. -
関連疾患
Microphthalmia, syndromic, 12 -
配列類似性
Belongs to the nuclear hormone receptor family. NR1 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
ドメイン
Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal ligand-binding domain. -
細胞内局在
Cytoplasm and Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5915 Human
- Entrez Gene: 218772 Mouse
- Entrez Gene: 24706 Rat
- Omim: 180220 Human
- SwissProt: P10826 Human
- SwissProt: P22605 Mouse
- Unigene: 543218 Human
- Unigene: 581530 Human
see all -
別名
- HAP antibody
- HBV-activated protein antibody
- NR1B2 antibody
see all
画像
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All lanes : Anti-Retinoic Acid Receptor beta antibody (ab53161) at 1/500 dilution
Lane 1 : HepG2 cell extract, untreated.
Lane 2 : HepG2 cell extract treated with the immunising peptide.
Predicted band size: 50 kDa
Observed band size: 50 kDa -
This image shows human breast carcinoma tissue stained with ab53161 at 1/50 dilution. The left hand image shows untreated tissue; the right hand image shows tissue treated with the immunising peptide.
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ICC/IF image of ab53161 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab53161, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (23)
ab53161 は 23 報の論文で使用されています。
- Jurmeister P et al. Mucosal melanomas of different anatomic sites share a common global DNA methylation profile with cutaneous melanoma but show location-dependent patterns of genetic and epigenetic alterations. J Pathol 256:61-70 (2022). PubMed: 34564861
- Tan M et al. Maternal vitamin A deficiency impairs cholinergic and nitrergic neurons, leading to gastrointestinal dysfunction in rat offspring via RARß. Life Sci 264:118688 (2021). PubMed: 33130074
- Kim KY et al. Downregulated miR-18b-5p triggers apoptosis by inhibition of calcium signaling and neuronal cell differentiation in transgenic SOD1 (G93A) mice and SOD1 (G17S and G86S) ALS patients. Transl Neurodegener 9:23 (2020). PubMed: 32605607
- Hinteregger B et al. Transgene integration causes RARB downregulation in homozygous Tg4-42 mice. Sci Rep 10:6377 (2020). PubMed: 32286473
- Khatib T et al. Decay in Retinoic Acid Signaling in Varied Models of Alzheimer's Disease and In-Vitro Test of Novel Retinoic Acid Receptor Ligands (RAR-Ms) to Regulate Protective Genes. J Alzheimers Dis 73:935-954 (2020). PubMed: 31884477