Anti-REST / NRSF 抗体 [CL0381] (ab211537)
Key features and details
- Mouse monoclonal [CL0381] to REST / NRSF
- Suitable for: IHC-P, ICC/IF
- Reacts with: Human
- Isotype: IgG1
製品の概要
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製品名
Anti-REST / NRSF antibody [CL0381]
REST / NRSF 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [CL0381] to REST / NRSF -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, ICC/IFmore details -
種交差性
交差種: Human -
免疫原
Recombinant fragment (proprietary-tag) corresponding to Human REST/ NRSF aa 850-1000.
Database link: Q13127 -
ポジティブ・コントロール
- Human tonsil, fallopian tube, testis, kidney, liver and cortex tissues.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 40% Glycerol (glycerin, glycerine), 59% PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
CL0381 -
アイソタイプ
IgG1 -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab211537の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use a concentration of 2 - 10 µg/ml.
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特記事項 |
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IHC-P
1/50 - 1/200. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use a concentration of 2 - 10 µg/ml. |
ターゲット情報
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機能
Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. -
組織特異性
Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. -
配列類似性
Contains 9 C2H2-type zinc fingers. -
細胞内局在
Nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 5978 Human
- Omim: 600571 Human
- SwissProt: Q13127 Human
- Unigene: 307836 Human
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別名
- Neural Restrictive Silencer Factor antibody
- Neural-restrictive silencer factor antibody
- Neuron Restrictive Silencer Factor antibody
see all
画像
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Immunocytochemistry/Immunofluorescence analysis of U-2 OS cells labelling REST with ab211537 at 10 µg/mL, showing specific staining in the nucleoplasm in green. Microtubules are visualized in red.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human liver tissue labeling REST / NRSF with ab211537 at 1/50 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human cortex tissue labeling REST / NRSF with ab211537 at 1/50 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labeling REST / NRSF with ab211537 at 1/50 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human testis tissue labeling REST / NRSF with ab211537 at 1/50 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human fallopian tube tissue labeling REST / NRSF with ab211537 at 1/50 dilution.
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human tonsil tissue labeling REST / NRSF with ab211537 at 1/50 dilutio.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab211537 は論文での使用が確認できていません。