Application
Western blot
Sample
Rat Tissue lysate - nuclear (Hippocampus, liver and MCF7 whole cell homogenates)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
80 µg
Specification
Hippocampus, liver and MCF7 whole cell homogenates
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
17 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBSTween (0.1%)
Dilution
1/300
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit HRP (Cell Signaling Technology)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Positive control
I used liver nuclear homogenates and MCF7 whole cell extracts
Additional data
Additional Notes
I recently received the anti-Rest/NRSF antibody (Ab21635). I've had troubles with band detection in my hippocampus samplesin WB analysis. So, I decided to use two positive controls (liver nuclear extract and MCF7 whole celll extract) and I haven't had any signal. I hope ypu can help me. I'm looking forward to your response!
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
Dr. Octavio Mercado-Gomez
Verified customer
投稿 May 12 2016